Enhanced tuburculocidal activity and decreased fumes from glutaraldehyde disinfectant using acetate salts and alcohol
View Patent ↗The addition of alcohol plus acetate salts unexpectedly enhanced the tuberculocidal activity of glutaraldehyde, and decreased the fumes of glutaraldehyde from the formula of a high-level disinfectant.
1. An aqueous, room temperature, low-fumes, disinfecting and/or sterilizing water-based solution having an activated pH of from 7.3 to about 7.9 comprising:
from about 2.0% to about 5.0% glutaraldehyde by volume;
from about 5.0% to about 26.0% alcohol by volume, wherein the alcohol is selected from the group consisting of methanol, ethanol, and isopropanol;
an effective amount of a buffer compatible with glutaraldehyde, wherein the buffer is phosphate buffer; and
from about 3% by weight to about 8% by weight of an acetate salt.
2. An aqueous disinfecting and/or sterilizing solution according to claim 1 wherein the alcohol concentration is 24% to about 26% by volume.
3. An aqueous disinfecting and/or sterilizing solution according to claim 2 wherein the acetate salt is present at about 8% by weight of the total solution.
4. An aqueous disinfecting and/or sterilizing solution according to claim 1 further including a surfactant selected from the group consisting of nonionic, cationic and anionic surfactants at a level of from 0.0025% to 0.01% by weight.
5. An aqueous disinfecting and/or sterilizing solution according to claim 4 wherein the surfactant is a nonionic surfactant.
6. An aqueous disinfecting and/or sterilizing solution according to claim 1 wherein the solution comprises above 2% to about 5% by volume of glutaraldehyde.
7. A disinfectant kit comprising:
a first container of storable solution that is stable for up to 12 months containing:
from about 2% to about 5% by volume of glutaraldehyde;
from about 5% to about 26% by volume of alcohol wherein the alcohol is selected from the group consisting of methanol, ethanol, and isopropranol; and
a sodium or potassium acetate salt of from about 3% by weight to about 8% by weight;
a second container of phosphate buffer in an amount sufficient to provide a concentration of 4 grams per liter to 7 grams per liter, and to provide a pH when combined with the first container of from 7.3 to 7.9; and
instructions for mixing said storable solution and said phosphate buffer to provide a working disinfecting solution that is stable for up to 14 days.
8. A disinfectant kit according to claim 7 , wherein the first container comprises above 2% to about 5% by volume of glutaraldehyde.
9. A disinfectant kit comprising:
a first container of storable solution that is stable for up to 12 months containing:
from about 2% to about 5% by volume of glutaraldehyde;
from 24% to about 26% by volume of alcohol wherein the alcohol is selected from the group consisting of methanol, ethanol, and isopropranol; and
an acetate salt of about 8% by weight;
a second container of phosphate buffer in an amount sufficient to provide a concentration of 4 grams per liter to 7 grams per liter, and to provide a pH when combined with the storable solution of from 7.3 to 7.9; and
instructions for mixing said storable solution and said phosphate buffer to provide a working disinfecting solution that is stable for up to 14 days.
10. A disinfectant kit according to claim 9 , wherein the first container comprises above 2% to about 5% by volume of glutaraldehyde.
11. A method of enhancing kill and decreasing fumes of glutaraldehyde disinfectant water based solution, comprising:
mixing from about 2.0% to about 5.0% glutaraldehyde by volume;
from about 5.0% to about 26.0% alcohol by volume, wherein the alcohol is selected from the group consisting of methanol, ethanol, and isopropanol; and an effective amount of a buffer compatible with glutaraldehyde, wherein the buffer is phosphate buffer; with from about 3% by weight to about 8% by weight of an acetate salt,
wherein the resultant mixture has an activated pH of from 7.3 to about 7.9.