Apparatus for characterizing and measuring the concentration of opaque particles within a fluid sample
View Patent ↗A system and method for counting opaque particles within a fluid sample. The system uses an optical lens system to focus a light beam onto a sample on a multi-dimensional translation stage. The translation stage is moved in a pattern such that the intensity of the transmitted light is measured as a function of path length. A photo detector is used to measure the transmitted light through the sample. An analog-to-digital converter quantifies the transmitted light intensity. Changes in light intensity along the path length are correlated with the detection of an opaque particle. Data processing algorithms are implemented to automatically determine the background noise level associated with the acquired data and to set a discriminator level above which a particle is registered. The total number of particles and an areal density is reported along with an estimate of the uncertainty.
1. A method for measuring concentration of opaque particles in a fluid, comprising the steps of:
a. providing an optical lens system comprising a translation stage, said translation stage having three axes of movement;
b. introducing a sample of said fluid onto said stage, said particles in said fluid having a range of size with diameter less than about 20 microns;
c. selecting a laser light source that produces a laser light beam with a wavelength effective to transmit through said fluid, but not said particles;
d. focusing said laser light beam into said sample with a beam waist of said light beam based on the size of said particles;
e. directing said light beam along a plurality of path lengths across a predetermined area of said sample;
f. detecting light transmitted through said sample along each said path length;
g. quantifying the intensity of said transmitted light; and
h. correlating said quantified transmitted light to a concentration of said particles in said sample.
2. The method of claim 1 wherein said correlating step comprises signal processing algorithms for filtering electronic and stochastic noise.
3. The method of claim 2 wherein a thickness of said sample varies within said predetermined area.
4. The method of claim 1 further comprising the step of determining said predetermined area so as to achieve a desired precision of results.
5. The method of claim 4 further comprising the step of increasing said predetermined area to reduce the effects of inhomogeneity of said sample.
6. The method of claim 1 wherein said translation stage is a flow cell.
7. The method of claim 1 wherein said beam waist has a cross dimension of approximately one micrometer.