Multiple marker characterization of single cells
Methods use concurrent multiple cellular probes conjugated to fluorescent compounds of different wavelengths to characterize single cells that have been isolated from a body fluid using density gradient centrifugation. Specific antibodies, peptides, nucleotides or oligonucleotides are used as probes for both identification and characterization of a single cell.
1 - 58 . (canceled)
59 . A method of characterizing single circulating epithelial cancer cells, other than prostate cancer cells, obtained from about 5 mL to 75 mL of blood comprising:
concurrently measuring multiple cellular markers using fluorescent probes, wherein said probes emit different wavelengths of light to distinguish multiple cellular markers expressed in said cells using fluorescence microscopy.