Purified polypeptides having IL-13 receptor activity
This invention relates to a purified polypeptide, comprising an amino acid sequence chosen from: a) the sequence SEQ ID No. 2, b) any biologically active sequence derived from SEQ ID No. 2.
1 - 43 . (canceled)
44 . A purified polypeptide comprising an amino acid sequence selected from the group consisting of:
(a) the amino acid sequence of SEQ ID NO:2,
(b) the amino acid sequence of SEQ ID NO:2 wherein the 8 c-terminal residues thereof are substituted with the 6 residues VRCVTL of SEQ ID NO: 11,
(c the amino acid sequence 1 to 343 of SEQ ID NO:2, and
(d) the amino acid sequence 1 to 337 of SEQ ID NO:2.
45 . A purified polypeptide comprising the amino acid sequence of SEQ ID NO:2.
46 . A purified polypeptide comprising the amino acid sequence of SEQ ID NO:2 wherein the 8 c-terminal residues thereof are substituted with the 6 residues VRCVTL of SEQ ID NO: 11.
47 . A purified polypeptide in soluble form comprising an amino acid sequence selected from the group consisting of:
(a) the amino acid sequence of SEQ ID NO:2 from residues 1 to 343 and
(b) the amino acid sequence of SEQ ID NO:2 from residues 1 to 337.
48 . An isolated IL-13bc (IL-13Rβ) protein comprising an amino acid sequence selected from the group consisting of:
(a) the amino acid sequence of SEQ ID NO:2,
(b) the amino acid sequence of SEQ ID NO:2 from amino acids 26 to 341,
(c) the amino acid sequence of SEQ ID NO: 2 from amino acids 363 to 380, and
(d) fragments of (a) to (c) which bind IL-13.
49 . The protein of claim 48 comprising the sequence from amino acid 26 to 341 of SEQ ID NO: 2.
50 . The protein of claim 48 comprising the sequence from amino acid sequence 363 to 380 of SEQ ID NO: 2.
51 . The protein of claim 48 wherein said amino acid sequence is part of a fusion protein.
52 . The isolated protein of claim 51 comprising an Fc fragment.
53 . A protein produced according to a process comprising:
(a) growing a culture of a host cell in a suitable culture medium and
(b) purifying the protein from the culture, wherein said host cell is transformed with a polynucleotide operably linked to an expression control sequence, and wherein said polynucleotide comprises a nucleotide sequence selected from the group consisting of:
a. a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:2;
b. a nucleotide sequence encoding the IL-13Rβ binding chain varying from the sequence of the nucleotide sequence encoding the amino acid sequence of SEQ ID NO: 2 as a result of degeneracy of the genetic code;
c. a nucleotide sequence capable of hybridizing under stringent conditions comprising hybridization at 52° C. in 5×SSC followed by washing at 52° C. in 2×SSC to a nucleotide sequence encoding the amino acid sequence of SEQ ID NO: 2.
54 . The protein of claim 53 wherein said nucleotide sequence is that of a nucleotide sequence encoding the amino acid sequence of SEQ ID NO: 2.
55 . The protein of claim 53 wherein said nucleotide sequence encodes the IL-13Rβ binding chain varying from the sequence encoding the amino acid sequence of SEQ ID NO: 2 as a result of the degeneracy of the genetic code.
56 . The protein of claim 53 wherein said nucleotide sequence is that of a nucleotide sequence capable of hybridizing under stringent conditions comprising hybridization at 52° C. in 5×SSC followed by washing at 52° C. in 2×SSC.
57 . The protein of claim 53 comprising SEQ ID NO: 2.
58 . An isolated IL-13Rβ protein comprising an amino acid sequence selected from the group consisting of:
(e) the amino acid sequence of SEQ ID NO:2,
(f) the amino acid sequence of SEQ ID NO:2 from amino acids 23 to 342,
(g) the amino acid sequence of SEQ ID NO: 2 from amino acids 365 to 380, and
(h) fragments of (a) to (c) which bind IL-13.
59 . The protein of claim 58 comprising the sequence from amino acid 23 to 342 of SEQ ID NO:2.
60 . The protein of claim 58 comprising the sequence from amino acid 365 to 380 of SEQ ID NO:2.
61 . An isolated IL-13Rβ protein comprising an amino acid sequence selected from the group consisting of:
(a) the amino acid sequence of SEQ ID NO: 2 from amino acids 23 to 380;
(b) the amino acid sequence of SEQ ID NO: 2 from amino acids 23 to 343;
(c) the amino acid sequence of SEQ ID NO: 2 from amino acids 364 to 380; and
(d) fragments of (a) to (c) which bind IL-13.
62 . A protein produced according to a process comprising:
(a) growing a culture of a host cell in a suitable culture medium and
(b) purifying the protein from the culture, wherein said host cell is transformed with a polynucleotide operably linked to an expression control sequence and wherein said polynucleotide comprises a nucleotide sequence selected from the group consisting of
a. the nucleotide sequence of SEQ ID NO:1 from nucleotide 53 to nucleotide 1192;
b. a nucleotide sequence encoding the IL-13Rβ binding chain varying from the sequence of SEQ ID NO: 1 from nucleotide 53 to nucleotide 1192 as a result of degeneracy of the genetic code,
c. a nucleotide sequence capable of hybridizing under stringent conditions comprising hybridization at 52° C. in 5×SSC followed by washing at 52° C. in 2×SSC to the nucleotide sequence of SEQ ID NO: 1 from nucleotide 53 to nucleotide 1192.
63 . The protein of claim 62 wherein said nucleotide sequence comprises the nucleotide sequence of SEQ ID NO: 1 from nucleotide 53 to nucleotide 1192.
64 . The protein of claim 62 wherein said nucleotide sequence comprises a nucleotide sequence encoding the IL-13Rβ binding chain varying from the sequence of SEQ ID NO: 1 from nucleotide 53 to nucleotide 1192 as a result of degeneracy of the genetic code.
65 . The protein of claim 62 wherein said nucleotide sequence is that of an nucleotide sequence capable of hybridizing under stringent conditions at 52° C. in 5×SSC followed by washing at 52° C. in 2×SSC.