It is an object of the present invention to provide a detection surface used for biosensors, in which non-specific adsorption is suppressed and the generation of pinholes is also suppressed. The present invention provides a biosensor, which comprises a substrate coated with a hydrophobic polymer having an alkyl group substituted with a fluorine atom.
1. A method for detecting or measuring a substance interacting with a physiologically active substance, which comprises a step of allowing a test substance to come into contact with a biosensor to the surface of which the physiologically active substance binds via a covalent bond, the biosensor comprising a substrate coated with a hydrophobic polymer having an alkyl group substituted with a fluorine atom in a side chain, wherein the substance interacting with the physiologically active substance is detected or measured by a non-electrochemical method.
2. A method for detecting or measuring a substance interacting with a physiologically active substance, which comprises a step of allowing a test substance to come into contact with a biosensor to the surface of which the physiologically active substance binds via a covalent bond, the biosensor comprising a substrate coated with a hydrophobic polymer having an alkyl group substituted with a fluorine atom in a side chain, wherein the substance interacting with the physiologically active substance is detected or measured by surface plasmon resonance analysis.