Media for recovery of microorganism in the presence of antibiotics
The present invention provides a media and a method of using such as media for the bioburden or sterility testing of antibiotics or environmental testing of antibiotic manufacturing areas. The medium contains one or more divalent or trivalent cation constituents, preferably of magnesium, calcium, aluminum and iron that allow for microorganism growth even in the presence of residual antibiotic.
1 . A medium for growing microorganisms in the presence of an antibiotic comprising a growth medium for one or more types of microorganisms and one or more constituents containing one or more cations selected from the group consisting of divalent and trivalent cations.
2 . The medium of claim 1 wherein the one or more cations are selected from the group consisting of magnesium, calcium, aluminum and iron.
3 . The medium of claim 1 wherein the one or more cations are selected from the group consisting of magnesium sulfate, magnesium chloride, calcium chloride, calcium citrate, aluminum sulfate and iron sulfate.
4 . The medium of claim 1 wherein the one or more cations are selected from the group consisting of magnesium and calcium.
5 . The medium of claim 1 wherein the one or more cations are selected from the group consisting of magnesium sulfate, magnesium chloride, calcium chloride and calcium citrate.
6 . The medium of claim 1 wherein the one or more cations are selected from the group consisting of divalent and trivalent cations of magnesium, calcium, aluminum and iron.
7 . The medium of claim 1 wherein the medium is in the form selected from the group consisting of gel or broth.
8 . The medium of claim 1 wherein the medium is selected from the group consisting of Soybean Casein Digest Broth, Soybean Casein Digest Agar, Fluid Thioglycollate Medium, Sabouraud Dextrose Agar, Mueller Hinton Broth, Mueller Hinton Agar, Nutrient Broth and Nutrient Agar.
9 . The medium of claim 1 wherein the medium is a gel.
10 . The medium of claim 1 wherein the medium is a broth.
11 . The medium of claim 1 wherein the medium is a broth selected from the group consisting of Soybean Casein Digest, Fluid Thioglycollate Medium, Mueller Hinton Broth and Nutrient Broth.
12 . The medium of claim 1 wherein the one or more cation constituents are in a range of from about 0.1M to about 0.5M.
13 . The medium of claim 1 wherein the one or more cation constituents are in a range of from about 0.2M to about 0.4M.
14 . The medium of claim 1 wherein the divalent cations constituents are in an amount of about 0.3M in the media.
15 . A process for determining the existence of microbes in the presence of antibiotics comprising the steps of providing a sample containing an antibiotic to be tested, a holder and a growth media within the holder wherein the media contains one or more cation constituents selected from the group consisting of divalent and trivalent cations of magnesium, calcium, aluminum and iron;
placing the sample in contact with the growth media and incubating the media for a pre-selected time at a pre-selected temperature and
viewing the media to determine the presence of any microorganisms.
16 . The process of claim 15 further comprising providing a filter, filtering the sample through the filter and placing the filter onto the growth media before incubation.
17 . The process of claim 15 further comprising providing a filter, filtering the sample through the filter and placing the filter onto the growth media before incubation and wherein the filter has a pore size of from about 0.1 micron to about 1.2 microns and is formed of a material selected from the group consisting of regenerated cellulose, mixed cellulose esters, cellulose acetate, cellulose nitrate, nitrocellulose, PVDF, nylons, polycarbonates, polysulfones, polyethersulfones, polyarylsulfones and polyphenylsulfones.
18 . The process of claim 15 wherein the sample is selected from the group consisting of a liquid and a powder dissolved in a liquid.
19 . The process of claim 15 wherein the sample is air from a manufacturing area of antibiotic manufacturing.
20 . The process of claim 15 wherein the filter/media are incubated for a period of time from about 0 to 7 days at a temperature from 20° C. to 54° C.
21 . The process of claim 15 wherein the filter/media are incubated for a period of time from about 7 to about 14 days at a temperature from 20° C. to 54° C.
22 . The process of claim 15 wherein the one or more cations constituents are in a range of from about 0.1M to about 0.5M.
23 . The process of claim 15 wherein the one or more cations constituents are in a range of from about 0.2M to about 0.4M.
24 . The process of claim 15 wherein the one or more cations constituents are in an amount of about 0.3M.
25 . The medium of claim 1 wherein the medium is a gel and the gel is agar based.
26 . The process of claim 15 wherein the viewing is selected from the group consisting of visual counting of the colony forming units or bio- or chemi- luminescent detection of the presence of a microbe constituent.
27 . The process of claim 15 wherein the viewing is by the detection of an agent used to indicate the presence of a microbe constituent.