Patent Application
App. No. 11/203,602
Oligonucleotide mediated nucleic acid recombination
Loading inventors, assignments & file history…
Abstract
Methods of recombining nucleic acids, including homologous nucleic acids, are provided. Families of gene shuffling oligonucleotides and their use in recombination procedures, as well as polymerase and ligase mediated recombination methods are also provided.
Claims (4)
1 - 104 . (canceled)
105 . A method of producing a library of chimeric nucleic acid molecules encoding full-length chimeric proteins, said method comprising:
(a) synthesizing a plurality of ligatable synthetic oligonucleotides corresponding to parental nucleic acid sequences encoding full-length proteins; and
(b) assembling the oligonucleotides in order by ligation to produce the library of chimeric nucleic acid molecules encoding the full-length chimeric proteins.
Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Feb 8, 2024
From: CODEXIS MAYFLOWER HOLDINGS, LLC
To: CODEXIS, INC.
Reel/Frame 066528/0932 →