Sample plate for matrix-assisted laser desorption and ionization mass spectrometry
View Patent ↗A method of making a sample plate for mass spectrometric analysis of a specimen is provided comprising applying a mask to a substrate to form at least one sample site. The sample plate comprises a substrate having an electrically conductive surface, a mask applied to the electrically conductive surface. The mask has a rougher surface than the substrate. The sample site comprises a central portion formed from the electrically conductive surface and a marginal portion formed from the mask where the marginal portion is more hydrophobic than the central portion.
1. A sample plate for mass spectrometric analysis of a specimen comprising:
a substrate having an electrically conductive surface;
a mask disposed upon said substrate includes at least one target area and a margin around each target area;
said mask has a rougher surface than said substrate; and
said margin is more hydrophobic than said surface.
2. The sample plate of claim 1 , further comprising a first target area and a second target area, said first target area having a larger surface area than said second target area.
3. The sample plate according to claim 1 , wherein said mask further includes a mask spot placed within said each target area.
4. The sample plate according to claim 3 , wherein said mask spot is disposed upon said substrate and placed within a surface area of said each target area.
5. The sample plate of claim 1 , wherein the rough surface comprises a texture for promoting crystallization of the specimen adjacent the electronically conductive surface.
6. The sample plate of claim 1 , wherein the substrate comprises a non-conductive material.
7. The sample plate of claim 1 , further comprising a central portion formed from said surface and said margin.
8. A sample plate for mass spectrometric analysis of a specimen comprising:
a substrate having an electrically conductive surface;
a mask disposed upon said substrate includes at least one target area, a margin around each target area, and a mask spot placed within said each target area;
said mask and said mask spot have a rougher surface than said substrate; and
said margin and said mask spot are more hydrophobic than said surface.
9. The sample plate according to claim 8 , wherein said target area includes an opening in said mask.
10. The sample plate according to claim 8 , further comprising a first target area and a second target area, said first target area having a larger surface area than said second target area.
11. The sample plate according to claim 8 , wherein said mask spot is disposed upon said substrate and placed within a surface area of said each target area.
12. The sample plate according to claim 8 , further comprising a central portion formed from said surface and said margin.
13. The sample plate according to claim 8 , further comprising a plurality of target areas having various sizes.
14. A sample plate for mass spectrometric analysis of a specimen comprising:
a substrate having an electrically conductive surface;
a mask disposed upon said substrate includes at least one target area and a margin around each target area;
said mask has a rougher surface than said substrate;
said margin is more hydrophobic than said surface;
a first target area and a second target area; and said first target area having a larger surface area than said second target area.
15. The sample plate according to claim 14 , wherein said mask further includes a mask spot placed within said each target area.
16. The sample plate according to claim 15 ; wherein said mask spot is dis-posed upon said substrate and placed within a surface area of said each target area.
17. The sample plate of claim 14 , wherein the rough surface comprises a texture for promoting crystallization of the specimen adjacent the electronically conductive surface.