Nucleic acid and amino acid sequences relating to Staphylococcus epidermidis for diagnostics and therapeutics
The invention provides isolated polypeptide and nucleic acid sequences derived from Staphylococcus epidermidis that are useful in diagnosis and therapy of pathological conditions; antibodies against the polypeptides; and methods for the production of the polypeptides. The invention also provides methods for the detection, prevention and treatment of pathological conditions resulting from bacterial infection.
1 . An isolated nucleic acid comprising a nucleotide sequence encoding a Staphylococcus epidermidis polypeptide of SEQ ID NO: 5314 or 5547.
2 . A recombinant expression vector comprising the nucleic acid of claim 1 operably linked to a transcription regulatory element.
3 . A cell comprising a recombinant expression vector of claim 2 .
4 . (canceled)
5 . An isolated nucleic acid comprising a nucleotide sequence encoding a S. epidermidis polypeptide or a fragment thereof, said nucleic acid selected from the group consisting of SEQ ID NO: 2477 and SEQ ID NO: 2710.
6 . A recombinant expression vector comprising the nucleic acid of claim 5 operably linked to a transcription regulatory element.
7 . A cell comprising a recombinant expression vector of claim 6 .
8 - 9 . (canceled)
10 . An isolated nucleic acid comprising a nucleotide sequence of at least 8 nucleotides in length, wherein the sequence is hybridizable to a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NOS: 2477 and 2710.
11 - 28 . (canceled)
29 . The isolated nucleic acid of claim 1 , wherein the nucleic acid comprises of SEQ ID NO: 2477.
30 . The isolated nucleic acid of claim 1 , wherein said nucleic acid consists of SEQ ID NO: 2477.
31 . The isolated nucleic acid of claim 1 , wherein said nucleic acid comprises SEQ ID NO: 2710.
32 . The isolated nucleic acid of claim 1 , wherein said nucleic acid consists of SEQ ID NO: 2710.
33 . A nucleic acid which has at least 90% homology to SEQ ID NO: 2477 or 2710.
34 . The nucleic acid of claim 5 , wherein the nucleic acid is DNA or RNA.
35 . A nucleic acid, wherein the nucleic acid is DNA, RNA, single-stranded antisense, or an RNA/DNA duplex comprising SEQ ID NO: 2477, a transcription product of SEQ ID NO: 2477, or an antisense strand of SEQ ID NO: 2477.
36 . A nucleic acid, wherein the nucleic acid is DNA, RNA, single-stranded antisense, or an RNA/DNA duplex comprising SEQ ID NO: 2710, a transcription product of SEQ ID NO: 2710, or an antisense strand of SEQ ID NO: 2710.