Biosensor electrophoresis
View Patent ↗The invention provides methods of detecting, purifying, quantifying, and separating molecules using an electrophoresis adapted biosensor.
1. An apparatus comprising:
(a) a colorimetric resonant label-free optical biosensor comprising:
(i) a one- or two-dimensional grating comprised of a high refractive index material deposited on a substrate comprising a low refractive index material;
(ii) a film of electrically conducting material on a top surface of the grating; and
(iii) one or more receptor molecules immobilized on the electrically conducting material;
(b) a gel suitable for separation of molecules,
(c) electrodes for applying a voltage to move any molecules in the gel to the one- or two-dimensional grating;
wherein the gel and the colorimetric resonant label-free biosensor are in contact with each other.
2. The apparatus of claim 1 , wherein the calorimetric resonant label-free biosensor is in the gel.
3. The apparatus of claim 1 , wherein the colorimetric resonant label-free biosensor is on the gel.
4. The apparatus of claim 1 , wherein the label-free biosensor is brought into contact with the gel after the molecules have been separated within the gel.
5. The apparatus of claim 1 , wherein the one- or two-dimensional grating is formed in the substrate material and a film of higher refractive index material is deposited on a top surface of the grating.
6. The apparatus of claim 1 , wherein the gel is selected from the group consisting of an agarose gel, a polyacrylamide gel, a polysaccharide agarose gel, a gradient gel, an isoelectric focusing gel, a sodium dodecyl sulfate (SDS) polyacrylamide gel, a two-dimensional electrophoresis gel, and combinations thereof.