Methods for determining susceptibility to dental caries
The present invention provides methods for determining whether a human being is susceptible to dental caries. The methods each include the steps of measuring the amount of α-defensins HNP 1, HNP 2 and HNP 3 in saliva obtained from a human being, and determining whether a reduced amount of the α-defensins HNP 1, HNP 2 and HNP 3 is present in the saliva, thereby determining whether the human being is susceptible to dental caries.
1. A method of determining whether a human child is susceptible to dental caries, the method comprising the steps of:
(a) measuring the amount of α-defensins HNP 1, HNP 2 and HNP 3 in saliva obtained from a human child; and
(b) comparing the measured amount of the α-defensins HNP 1, HNP 2 and HNP 3 to a reference value, wherein an amount less than a reference value of 0.4 μg of HNP 1, HNP 2 and HNP 3 per milliliter of saliva indicates the human child is susceptible to dental caries.
2. A method of claim 1 , wherein the amount of α-defensins HNP 1, HNP 2 and HNP 3 is measured using a method selected from the group consisting of enzyme linked immunoassay, mass spectrometry, high pressure liquid chromatography, dot immunoassay and a combination of gel electrophoresis and western blot.
3. A method of claim 1 , wherein the amount of α-defensins HNP 1, HNP 2 and HNP 3 is measured using an enzyme linked immunoassay.
4. A method of claim 1 , wherein the α-defensins HNP 1, HNP 2 and HNP 3 are each at least 90% identical to an HNP 1 α-defensin peptide consisting of the amino acid sequence set forth in SEQ ID NO:1.
5. A method of claim 1 , wherein the α-defensins HNP 1, HNP 2 and HNP 3 are each at least 95% identical to an HNP 1 α-defensin peptide consisting of the amino acid sequence set forth in SEQ ID NO:1.
6. A method of claim 1 , wherein the α-defensins HNP 1, HNP 2 and HNP 3 are each at least 99% identical to an HNP 1 α-defensin peptide consisting of the amino acid sequence set forth in SEQ ID NO:1.
7. A method of claim 1 , wherein an amount of the HNP 1, HNP 2 and HNP 3 peptides less than 0.3 μg per milliliter saliva indicates susceptibility of the human child to dental caries.
8. A method of claim 1 , wherein an amount of the HNP 1, HNP 2 and HNP 3 peptides less than 0.2 μg per milliliter saliva indicates susceptibility of the human child to dental caries.
9. A method of claim 1 , wherein an amount of the HNP 1, HNP 2 and HNP 3 peptides less than 0.1 μg per milliliter saliva indicates susceptibility of the human child to dental caries.
10. A method of claim 1 , wherein an amount of the HNP 1, HNP 2 and HNP 3 peptides less than 0.3 μg per milligram total salivary protein indicates susceptibility of the human child to dental caries.
11. A method of claim 1 , wherein an amount of the HNP 1, HNP 2 and HNP 3 peptides less than 0.2 μg per milligram total salivary protein indicates susceptibility of the human child to dental caries.
12. A method of claim 1 , wherein an amount of the HNP 1, HNP 2 and HNP 3 peptides less than 0.1 μg per milligram total salivary protein indicates susceptibility of the human child to dental caries.
13. A method of claim 1 , wherein the saliva obtained is unstimulated saliva.