Systems and methods for isolating stromal cells from adipose tissue and uses thereof
Methods of isolating cells from adipose tissue that have potential to differentiate into cells of mesenchymal origin, including cells of chondrogenic, osteogenic, adipogenic, and/or myogenic origin, comprising: (a) subjecting adipose tissue to an electromagnetic, sonic, or other wave energy source; and (b) centrifuging the tissue to form a pellet comprising stem cells. In various embodiments, the method is carried out without any enzymatic digestion of the adipose tissue. In other embodiments, the method additionally comprises enzymatically digesting the tissue. In various embodiments, methods comprise subjecting the tissue to ultrasonic energy. In some embodiments, the method does not comprise enzymatic digestion of the adipose tissue. In other embodiments, the method additionally comprises enzymatically digesting the tissue. Methods are also provided for intraoperative harvest and delivery of autologous stem cells to the site of acute or chronic wounds. In one embodiment, an autologous supply of stem cells is provided for operative use.
1 . A method for isolating or recovering adult stem cells from adipose tissue composing:
subjecting adipose tissue to an electromagnetic or sonic energy source; and
centrifuging the tissue to form a pellet comprising stem cells.
2 . A method according to claim 1 , wherein the method does not comprise an enzymatic digestion step.
3 . A method according to claim 1 , additionally comprising enzymatic digestion of the tissue.
4 . A method according to claim 1 , wherein the energy source is electromagnetic.
5 . A method according to claim 1 , wherein the energy source is sonic.
6 . A method for preparing autologous stem cells from a human patient comprising:
removing adipose tissue by liposuction;
exposing the removed tissue to ultrasound; and
centrifuging the sonicated tissue to form a pellet comprising the stem cells.
7 . A method according to claim 6 , wherein the method does not comprise an enzymatic digestion step.
8 . A method according to claim 6 , additionally comprising enzymatic digestion of the tissue.
9 . An intraoperative method for treating a human or other animal subject having a chronic or acute soft tissue injury comprising:
removing fat tissue from the subject;
exposing the fat tissue to ultrasonic energy;
centrifuging the sonicated tissue to form a pellet comprising multipotent cells;
suspending the pellet in a liquid matrix; and
applying the suspended pellet to the site of the injury.
10 . A method according to claim 9 , wherein the liquid matrix comprises saline.
11 . A method according to claim 10 , wherein the liquid matrix comprises phosphate-buffered saline.
12 . A method according to claim 9 , wherein the liquid matrix comprises concentrated blood platelets.
13 . A method according to claim 9 , wherein the liquid matrix comprises blood.
14 . A method according to claim 9 , wherein the liquid matrix comprises serum or plasma.
15 . A method according to claim 9 , wherein the injury is an acute surgical wound.
16 . A method according to claim 9 , wherein removing the fat tissue comprises liposuction.
17 . A method according to claim 9 , wherein all of the steps are carried out on the subject during a single operation.
18 . A method according to claim 9 , wherein the subject is human.
19 . A composition for tissue construction in a human or other animal subject, comprising:
(a) adipose derived stem cells; and
(b) a biocompatible carrier;
wherein said adipose derived stem cells are derived by application of electromagnetic or sonic energy to adipose tissue.
20 . A method according to claim 19 , wherein the energy source is ultrasonic.