Humanized anti-CD4 antibody with immunosuppressive properties
A humanized antibody derived from mouse monoclonal anti-CD4 antibody B-F5 is able to activate CD25+CD4+ regulatory T cells and is useful for preparing immunosuppressive compositions.
1. A humanized hB-F5 antibody derived from mouse monoclonal anti-CD4 antibody B-F5, wherein said hB-F5 antibody comprises V domains comprising:
a) an H chain V domain comprising the sequence, EEQLVESGGGLVKPGGSLRLSCAASGFSFSDCRMYWLRQAPGKGLEWIGVISVK SENYGANYAESVRGRFTISRDDSKNTVYLQMNSLKTEDTAVYYCSASYYRYDV GAWFAYWGQGTLVTVSS (SEQ ID NO: 1); and
b) a L chain V domain comprising the sequence: DIVMTQSPDSLAVSLGERATINCRASKSVSTSGYSYIYWYQQKPGQPPKLLIYLAS ILESGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQHSRELPWTFGQGTKVEIK (SEQ ID NO: 2),
wherein said bB-F5 antibody is able to activate a subset of T CD4 + cells comprising CD4 + CD25 + cells.
2. A fragment of the hB-F5 antibody of claim 1 , wherein said fragment comprises the V domains of SEQ ID NO: I and SEQ ID NO: 2.
3. A therapeutic composition comprising a humanized antibody of claim 1 , or a fragment of the hB-F5 antibody of claim 1 , wherein said fragment comprises the V domains of SEQ ID NO: I and SEQ ID NO: 2.
4. A method comprising:
preparing an immunosuppressive composition comprising the humanized antibody of claim 1 , or a fragment of the hB-F5 antibody of claim 1 ;
wherein said fragment comprises the V domains of SEQ ID NO: 1 and SEQ ID NO: 2.