Absorption spectrometric analysis microchip and method

1. Absorption spectrometric analysis microchip, comprising a microchip body in which is formed:

a chamber for holding a sample,

a chamber for holding a reagent which reacts with the sample,

a mixing chamber for mixing the reagent with the sample to form a mixture, and

a sensing part having a mixture receiving sensing chamber for holding the mixture, the sensing part having a light incidence surface for the entry of light into the sensing chamber on first face thereof and a light exit surface for emergence of light from the mixture receiving sensing chamber on an opposite second face thereof,

wherein the sensing part with the sensing chamber is located in a protrusion of the microchip body,

wherein the light incidence surface is located in a first recess area of the sensing part and light exit surface is located in a second recess area of the sensing part, and

wherein the sensing part has a longitudinal axis that is essentially aligned with the optical axis of the light that is directed through the sensing chamber during at least one of an absorption and a scattering of the light measurement, the first and second recesses being located opposite one another in the direction of said optical axis,

wherein the light incidence surface is formed by a portion of the microchip body itself and is located in a first recessed area of the sensing part and the light exit surface is formed by a portion of the microchip body itself and is located in a second recessed area of the sensing part such that damage to the light incidence and exit surfaces and adherence of impurities to the light incidence and exit surfaces is prevented.

2. Absorption spectrometric analysis microchip in accordance with claim 1 , wherein the light incidence surface and light exit surface are located on opposite edge areas of the protrusion of the microchip body with respect to a direction of propagation of the light.

3. Absorption spectrometric analysis microchip in accordance with claim 1 , wherein each recess area comprises a recess in a respective side area of the microchip body.

4. Absorption spectrometric analysis microchip in accordance with claim 3 , wherein the recess is a rectanguloidal recess.

5. Absorption spectrometric analysis microchip in accordance with claim 1 , wherein said chambers are formed by facing surfaces of a pair of plate components that are bonded together.

6. Microchip in accordance with claim 1 , wherein the chamber for holding a sample comprises a first and a second sample chamber which are connected to one another via a channel, wherein the chamber for holding a reagent comprises a first and a second reagent chamber, wherein the mixing chamber is connected by channels to the second sample chamber and the reagent chambers, wherein a channel leads from the mixing chamber to the mixture receiving sensing chamber, and wherein a suction channel leads out of the mixture receiving sensing chamber for connection to a suction device.

7. Absorption spectrometric analysis microchip unit, comprising, an absorption spectrometric analysis microchip, comprising a microchip body in which is formed:

a first chamber for holding a sample,

a second chamber for holding a reagent which reacts with the sample,

a mixing chamber for mixing the reagent with the sample to for a mixture, and

a sensing part having a mixture receiving sensing chamber for holding the mixture, the sensing part having a light incidence surface for the entry of light into the sensing chamber on first face thereof and a light exit surface for emergence of light from the mixture receiving sensing chamber on an opposite second face thereof,

wherein the sensing part with the sensing chamber is located in a protrusion of the microchip body,

wherein the light incidence surface is formed by a portion of the microchip body itself and is located in a first recess area of the sensing part and light exit surface is formed by a portion of the microchip body itself and is located in a second recess area of the sensing part, and wherein the sensing part has a longitudinal axis that is essentially aligned with an optical axis of a light source that is directed through the sensing chamber during at least one of an absorption and a scattering of the light measurement, the first and second recesses being located opposite one another in the direction of said optical axis, and

a chip holder in which the microchip is accommodated with the light incidence surface resting against an end of a capillary passage of the chip holder and with the light exit surface resting against a light receiving component of the chip holder.

8. A method of conducting an absorption spectrometric analysis with a microchip unit in accordance with claim 7 for quantitative analysis of enzymes in blood or blood plasma comprising the steps of:

introducing a blood sample and a reagent into said first and second chambers, respectively;

mixing said blood sample and said reagent together in said mixing chamber;

receiving the mixture produced by said mixing step into said sensing chamber;

accommodating said chip into said chip holder;

aligning an optical axis of a light source with said light incidence surface at one lateral side of the protrusion and along the longitudinal axis of said sensing part;

introducing light from said light source into said light incidence surface in the first recess area on the first face of the sensing chamber;

receiving light, at an opposite second lateral side of the protrusion, from said light exit surface in the second recess area on the opposite second face of the sensing chamber, and

measuring at least one of light absorbance and scattering of the light characteristics of the received light.