Methods of using sulfur nucleophiles as improved alternatives to sodium bisulfite for methylated DNA analysis
The invention provides for the use of sulfur nucleophiles in analyzing methylated DNA and novel sulfur nucleophiles suitable for such us.
1 . A method for converting cytosine to uracil in a nucleic acid comprising the steps of:
providing a nucleic acid comprising at least one cytosine nucleobase; and
reacting said nucleic acid with a nucleophilic organo-sulfur compound.
2 . The method of claim 1 wherein said nucleophilic organo-sulfur compound is a compound of Formula I:
wherein
R 1 and R 2 are each independently selected from the group consisting of hydroxyl, alkyl, aryl, amino, alkoxy, and aryloxy, each of which may be optionally substituted; and
or R 1 and R 2 can be concatenated to form a 4-8 membered ring optionally having 1 or 2 additional hetero ring atoms selected from N, S, and O, wherein said ring can be optionally substituted with one or more substituents;
or a salt thereof.
3 . The method of claim 2 wherein said amino of said R 1 and said R 2 has the formula NR 3 R 4 , and said alkoxy of said R 1 and said R 2 has the formula OR 5 ; and
wherein R 3 , R 4 and R 5 are each independently selected from the group consisting of hydrogen, methyl, ethyl, propyl, isopropyl, 2-hydroxyethyl, and 2-methoxyethyl.
4 . The method of claim 2 wherein said organo-sulfur compound is a salt of formula I where one of R 1 and R 2 forms an ionic bond with a cation selected from lithium, sodium, magnesium and ammonium.
5 . The method of claim 2 , wherein said reacting step is carried out with a mixture comprising a bisulfite ion and said nucleophillic organo-sulfur compound.
6 . A method for assessing the methylation status of cytosine comprising the steps of:
providing a sample nucleic acid comprising at least one cytosine nucleobase of unknown methylation status; and
reacting said nucleic acid with a nucleophilic organo-sulfur compound comprising a radio-labeled substituent.
7 . The method of claim 7 wherein said nucleophilic organo-sulfur compound is a compound of formula I:
wherein
R 1 and R 2 are each independently selected from the group consisting of hydroxyl, alkyl, aryl, amino, alkoxy, and aryloxy, and a radiolabel substituent, wherein each of said alkyl, aryl, amino, alkoxy, and aryloxy can be optionally substituted;
wherein at least one of R 1 and R 2 comprises a radio-labeled substituent;
or a salt thereof.
8 . The method of claim 8 further comprising the steps of:
providing a control nucleic acid comprising at least one cytosine nucleobase of known non-methylated status;
reacting said nucleic acid with the same said nucleophilic organo-sulfur compound; and
comparing the level of radioactivity of the sample and control to determine the relative content of methylated cytosine in the sample based on the rates of reaction of the methylated cytosine and unmethylated cytosine.
9 . The method of claim 8 wherein said amino of said R 1 and said R 2 has the formula NR 3 R 4 , and said alkoxy of said R 1 and said R 2 has the formula OR 5 ; and
wherein R 3 , R 4 and R 5 are each independently selected from the group consisting of hydrogen, methyl, ethyl, propyl, isopropyl, 2-hydroxyethyl, and 2-methoxyethyl.
10 . The method of claim 8 wherein said organo-sulfur compound is a salt of formula I where one of R 1 and R 2 forms an ionic bond with a cation selected from lithium, sodium, magnesium and ammonium.
11 . The method of claim 8 , wherein said reacting step is carried out with a mixture comprising a bisulfite ion and said nucleophillic organo-sulfur compound.
12 . A method for assessing the methylation status of cytosine comprising the steps of:
providing a sample nucleic acid comprising at least one cytosine nucleobase of unknown methylation status; and
reacting said nucleic acid with a nucleophilic organo-sulfur compound comprising a fluorescent or chemiluminescent moiety.
13 . The method of claim 13 wherein said nucleophilic organo-sulfur compound is a compound of formula I:
wherein
R 1 and R 2 are each independently selected from the group consisting of hydroxyl, alkyl, aryl, amino, alkoxy, and aryloxy, and a radiolabel substituent, wherein each of said alkyl, aryl, amino, alkoxy, and aryloxy can be optionally substituted;
wherein at least one of R 1 and R 2 comprises a fluorescent or chemiluminescent moiety;
or a salt thereof.
14 . The method of claim 14 wherein said amino of said R 1 and said R 2 has the formula NR 3 R 4 , and said alkoxy of said R 1 and said R 2 has the formula OR 5 ; and
wherein R 3 , R 4 and R 5 are each independently selected from the group consisting of hydrogen, methyl, ethyl, propyl, isopropyl, 2-hydroxyethyl, and 2-methoxyethyl.
15 . The method of claim 14 wherein said organo-sulfur compound is a salt of formula I where one of R 1 and R 2 forms an ionic bond with a cation selected from lithium, sodium, magnesium and ammonium.
16 . The method of claim 14 wherein said radio-labeled substituents comprises one of 3 H and 14 C.
17 . The method of claim 14 further comprising the steps of:
providing a control nucleic acid comprising at least one cytosine nucleobase of known non-methylated status;
reacting said control nucleic acid with the same said nucleophilic organo-sulfur compound; and
detecting and comparing the level of optical activity of the sample and control to determine the relative content of methylated cytosine.
18 . The method of claim 14 , wherein said reacting step is carried out with a mixture comprising a bisulfite ion and said nucleophillic organo-sulfur compound.