Transgenic transchromosomal rodents for making human antibodies
View Patent ↗The present invention provides novel transgenic nonhuman mammals capable of producing human sequence antibodies, as well as methods of producing and using these antibodies.
1. A transgenic mouse comprising two human immunoglobulin loci, wherein one human immunoglobulin locus is a human heavy chain locus located on an autonomous transchromosome comprising a fragment of human chromosome 14 having a human chromosome 14 centromere, and the other human immunoglobulin locus is a human light chain locus transgene integrated into the mouse genome, wherein the transgenic mouse expresses a detectable amount of a human immunoglobulin.
2. The transgenic mouse of claim 1 wherein the transchromosome is the SC20 transchromosome.
3. The transgenic mouse of claim 1 wherein the endogenous mouse heavy chain locus and at least one endogenous mouse light chain locus are inactivated.
4. The transgenic mouse of claim 1 wherein the endogenous mouse heavy chain locus and an endogenous mouse kappa light chain locus are inactivated.
5. The transgenic mouse of claim 1 wherein at least a part of the human light chain locus transgene is located on a YAC vector.
6. The transgenic mouse of claim 1 wherein the transgene is the KCo5 transgene.
7. The transgenic mouse of claim 1 wherein the human heavy chain locus is located on transchromosome SC20 and the human light chain locus transgene is the human kappa light chain transgene KCo5.