Methods and apparatus for accurately positioning a device within the subgerminal cavity of avian eggs
View Patent ↗Methods an apparatus for accurately and reliably positioning a device within an egg are provided. An opening is formed within a portion of the shell of an egg and a device is extended through the opening. The device includes a needle having a lumen containing a fluid under pressure. Fluid pressure is monitored and, in response to detecting a change in pressure of the fluid, movement of the device is stopped. Alternatively, fluid flow is monitored and, in response to detecting fluid flow from the needle, movement of the device is stopped. The device may be a substance delivery device that is configured to deliver a substance into the subgerminal cavity of the egg. The device may be a substance removal device that is configured remove a substance from an egg.
1. A method of positioning a device within an egg, comprising:
forming an opening within a portion of the shell of the egg;
extending a device through the opening and into the egg, wherein the device comprises a needle having a lumen containing a fluid under pressure;
detecting a change in pressure of the fluid within the lumen; and
stopping the movement of the device in response to detecting a change in pressure of the fluid within the lumen.
2. The method of claim 1 , wherein the device comprises a substance delivery device and further comprising injecting a substance into the egg via the substance delivery device in response to stopping movement of the device.
3. The method of claim 2 , wherein the substance is selected from the group consisting of cells, vaccines, nucleic acids, proteins, peptides, viruses, antigens, hormones, growth factors, and cytokines.
4. The method of claim 3 , further comprising incubating the egg until hatch.
5. The method of claim 1 , wherein the device comprises a substance removal device and further comprising removing a substance from the egg via the substance removal device in response to stopping movement of the device.
6. The method of claim 1 , further comprising:
removing the device from the egg; and
sealing the opening in the egg shell.
7. The method of claim 5 , wherein the removing step is performed in response to detecting that pressure of the fluid in the lumen has fallen to a predetermined level.
8. A method of positioning a substance delivery device within the subgerminal cavity of an avian egg, comprising:
forming an opening within a portion of the shell of the egg;
extending a substance delivery device through the opening and into albumen within the egg, wherein the substance delivery device comprises a needle having a lumen containing a fluid under pressure, and wherein the lumen comprises an outlet;
detecting a change in pressure of the fluid within the lumen; and
stopping movement of the substance delivery device through the albumen such that the lumen outlet is positioned within the subgerminal cavity in response to detecting a change in pressure of the fluid within the lumen.
9. The method of claim 8 , further comprising injecting a substance into the subgerminal cavity via the substance delivery device in response to stopping movement of the device.
10. The method of claim 9 , wherein the substance is selected from the group consisting of cells, vaccines, nucleic acids, proteins, peptides, viruses, antigens, hormones, growth factors, and cytokines.
11. The method of claim 8 , further comprising:
removing the substance delivery device from the egg; and
sealing the opening in the egg shell.
12. The method of claim 11 , further comprising incubating the egg until hatch.
13. The method of claim 11 , wherein the removing step is performed in response to detecting that pressure of the fluid in the lumen has fallen to a predetermined level.
14. A method of positioning a device within an egg, comprising:
forming an opening within a portion of the shell of the egg;
extending a device through the opening and into the egg, wherein the device comprises a needle having a lumen containing a fluid under pressure, and wherein the lumen comprises an outlet;
detecting flow of the fluid through the lumen outlet; and
stopping movement of the device in response to detecting fluid flow.
15. The method of claim 14 , wherein the device comprises a substance delivery device and further comprising injecting a substance into the egg via the substance delivery device in response to stopping movement of the device.
16. The method of claim 15 , wherein the substance is selected from the group consisting of cells, vaccines, nucleic acids, proteins, peptides, viruses, antigens, hormones, growth factors, and cytokines.
17. The method of claim 14 , wherein the device comprises a substance removal device and further comprising removing a substance from the egg via the substance removal device in response to stopping movement of the device.
18. The method of claim 14 , further comprising:
removing the device from the egg; and
sealing the opening in the egg shell.
19. The method of claim 18 , further comprising incubating the egg until hatch.
20. A method of positioning a substance delivery device within the subgerminal cavity of an avian egg, comprising:
forming an opening within a portion of the shell of the egg;
extending a substance delivery device through the opening and into albumen within the egg, wherein the substance delivery device comprises a needle having a lumen containing a fluid under pressure, and wherein the lumen comprises an outlet;
detecting flow of the fluid through the lumen outlet; and
stopping movement of the substance delivery device through the albumen such that the lumen outlet is positioned within the subgerminal cavity in response to detecting fluid flow.
21. The method of claim 20 , further comprising injecting a substance into the subgerminal cavity via the substance delivery device in response to stopping movement of the device.
22. The method of claim 21 , wherein the substance is selected from the group consisting of cells, vaccines, nucleic acids, proteins, peptides, viruses, antigens, hormones, growth factors, and cytokines.
23. The method of claim 20 , further comprising:
removing the substance delivery device from the egg; and
sealing the opening in the egg shell.
24. The method of claim 23 , further comprising incubating the egg until hatch.
25. An apparatus configured for use with an egg, comprising:
an extendable and retractable needle, wherein the needle comprises a lumen containing fluid under pressure; and
a pressure sensing system comprising a pressure transducer configured to detect a change in pressure of the fluid within the lumen as the needle moves through material in an egg, wherein the pressure sensing system is configured to stop movement of the needle in response to the pressure transducer detecting a change in pressure of the fluid.
26. The apparatus of claim 25 , wherein the needle comprises a substance delivery device.
27. The apparatus of claim 25 , wherein the needle comprises a substance removal device.
28. The apparatus of claim 25 , further comprising a punch configured to form an opening in the shell of an egg.
29. An apparatus configured for use with an egg, comprising:
an extendable and retractable needle, wherein the needle comprises a lumen containing fluid under pressure; and
a flow sensing system comprising a flow sensor configured to detect flow of fluid through the lumen as the needle moves through material in an egg, wherein the flow sensing system is configured to stop movement of the needle in response to the flow sensor detecting fluid flow.
30. The apparatus of claim 29 , wherein the needle comprises a substance delivery device.
31. The apparatus of claim 29 , wherein the needle comprises a substance removal device.
32. The apparatus of claim 29 , further comprising a punch configured to form an opening in the shell of an egg.
33. The apparatus of claim 29 , wherein the flow sensor comprises a gas mass flow sensor.