System and method for finding regions of interest for microscopic digital montage imaging
View Patent ↗Imaging apparatuses and methods that capture a low-resolution image of a slide, identify a region of interest, and capture a high-resolution image of the region of interest.
1. An imaging apparatus, comprising:
a macro camera to capture a low-resolution digital image of a slide;
a system to identity a region of interest from the low-resolution digital image and to provide an indication of which regions should be imaged; and
a microscopic camera to capture a high-resolution image of the region of interest;
wherein identifying a region of interest includes differentiating the region of interest from a region not of interest based, at least in part, on the typical-location of tissue and non-tissue.
2. The imaging apparatus of claim 1 , wherein the macro camera is to capture at least two low-resolution digital images of the slide.
3. The imaging apparatus of claim 1 , wherein the system is to automatically identify the region of interest from the low-resolution digital image.
4. The imaging apparatus of claim 1 , wherein the system includes software executed by a computer.
5. The imaging apparatus of claim 1 , wherein the region of interest is a region containing tissue.
6. The imaging apparatus of claim 1 , wherein the microscopic camera is to capture a high-resolution image of at least two tissue containing regions.
7. The imaging apparatus of claim 1 , wherein identifying a region of interest includes differentiating the region of interest from a region not of interest based, at least in part, on the typical appearance of tissue and non-tissue.
8. The imaging apparatus of claim 1 , wherein providing an indication of which regions should be imaged includes providing a tiling matrix.
9. The imaging apparatus of claim 8 , the system is further to transpose the tiling matrix into coordinates of the high-resolution image to be captured.
10. An imaging apparatus, comprising:
a motorized stage having a slide holder;
a macro camera directed toward the slide holder to capture a low resolution digital image of a slide placed on the slide holder;
a system to identify a region of interest from the low-resolution digital image; and
a microscopic camera directed toward the slide holder through a microscopic optic to capture a high-resolution image of the region of interest;
wherein identifying a region of interest includes differentiating the region of interest from a region not of interest based, at least in part, on the typical location of tissue and non-tissue.
11. The imaging apparatus of claim 10 , wherein the system is to automatically identify the region of interest from the low-resolution digital image.
12. An imaging apparatus, comprising:
a camera directed toward a slide holder; and
a computer to direct the camera to capture a low-resolution digital image of a slide present on the slide holder, to identify at least two non-contiguous regions of interest from the low-resolution digital image, to provide an indication of which regions should be imaged, and to direct the camera to capture high-resolution images of the at least two non-contiguous regions of interest;
wherein identifying at least two non-contiguous regions of interest includes differentiating regions of interest from regions not of interest based, at least in part, on the typical location of tissue and non-tissue.
13. The imaging apparatus of claim 12 , wherein the camera is to capture at least two low-resolution digital images of the slide.
14. The imaging apparatus of claim 12 , wherein the computer is to automatically identify the at least two regions of interest from the low-resolution digital image.
15. The imaging apparatus of claim 12 , wherein the at least two regions of interest are tissue containing regions of the slide.
16. The imaging apparatus of claim 12 , wherein identifying at least two non-contiguous regions of interest includes differentiating regions of interest from regions not of interest based, at least in part, on the typical appearance of tissue and non-tissue.
17. The imaging apparatus of claim 12 , wherein providing an indication of which regions should be imaged includes providing a tiling matrix.
18. The imaging apparatus of claim 17 , wherein the computer is further to transpose the tiling matrix into coordinates of the high-resolution image to be captured.
19. An imaging method, comprising
capturing a low-resolution digital image of a slide;
identifying a region of interest from the low-resolution digital image by using a computer system;
providing an indication of which regions should be imaged; and
capturing a high-resolution image of the region of interest;
wherein identifying a region of interest includes differentiating the region of interest from a region not of interest based, at least in part, on the typical location of tissue and non-tissue.
20. The imaging method of claim 19 , further comprising capturing at least two low-resolution digital images of the slide.
21. The imaging method of claim 19 , wherein identifying the region of interest from the low-resolution digital image is performed automatically by a computer.
22. The imaging method of claim 19 , wherein the region of interest is a region containing tissue.
23. The imaging method of claim 19 , further comprising capturing high resolution images of at least two tissue containing regions.
24. The imaging method of claim 19 , wherein identifying a region of interest includes differentiating the region of interest from a region not of interest based, at least in part, on the typical appearance of tissue and non-tissue.
25. The imaging method of claim 19 , wherein providing an indication of which regions should be imaged includes providing a tiling matrix.
26. The imaging method of claim 25 , further comprising transposing the tiling matrix into coordinates of the high-resolution image to be captured.
27. An imaging method, comprising:
capturing a low-resolution digital image of a slide;
identifying at least two non-contiguous regions of interest from the low-resolution digital image by using a computer system;
providing an indication of which regions should be imaged; and
capturing high-resolution images of the at least two non-contiguous regions of interest;
wherein identifying at least two noncontiguous regions of interest includes differentiating regions of interest from regions not of interest based, at least in part, on the typical location of tissue and non-tissue.
28. The imaging method of claim 27 , further comprising capturing at least two low-resolution digital images of the slide.
29. The imaging method of claim 27 , wherein identifying the at least two regions of interest from the low-resolution digital image is performed automatically by a computer.
30. The imaging method of claim 27 , wherein the at least two regions of interest are tissue containing regions of the slide.
31. The imaging method of claim 27 , wherein identifying at least two noncontiguous regions of interest includes differentiating regions of interest from regions not of interest based, at least in part, on the typical appearance of tissue and non-tissue.
32. The imaging method of claim 27 , wherein providing an indication of which regions should be imaged includes providing a tiling matrix.
33. The imaging method of claim 32 , further comprising transposing the tiling matrix into coordinates of the high-resolution image to be captured.