Bisulfite conversion reagent
View Patent ↗Disclosed, among other things, are packaged bisulfite solutions comprising bisulfite reagent in an oxygen-impermeable container and methods.
1. A kit containing a pre-packaged bisulfite solution suitable for use in a DNA methylation analysis reaction involving the conversion of cytosine to uracil wherein said pre-packaged bisulfite solution comprises an aqueous deoxygenated solution of a bisulfite reagent enclosed within an oxygen-impermeable container such that the pre-packaged bisulfite solution maintains a bisulfite concentration that is at least 90% of the original concentration and useable in the DNA methylation analysis reaction for a period of approximately six months after packaging in said kit.
2. The pre-packaged bisulfite solution of claim 1 , wherein the bisulfite reagent comprises at least one counter-ion selected from a monovalent ion, a divalent ion and combinations thereof.
3. The pre-packaged bisulfite solution of claim 2 , wherein the counter-ion comprises a monovalent ion.
4. The pre-packaged bisulfite solution of claim 2 , wherein the counter-ion comprises a monovalent ion selected from sodium, lithium, potassium, ammonium, tetraalkylammonium and combinations thereof.
5. The pre-packaged bisulfite solution of claim 4 , wherein the counter-ion comprises sodium.
6. The pre-packaged bisulfite solution of claim 4 , wherein the counter-ion comprises lithium.
7. The pre-packaged bisulfite solution of claim 4 , wherein the counter-ion comprises potassium.
8. The pre-packaged bisulfite solution of claim 4 , wherein the counter-ion comprises ammonium.
9. The pre-packaged bisulfite solution of claim 4 , wherein the counter-ion comprises tetraalkylammonium.
10. The pre-packaged bisulfite of claim 2 , wherein the counter-ion comprises a divalent ion.
11. The pre-packaged bisulfite solution of claim 2 , wherein the counter-ion comprises a divalent ion selected from magnesium, manganese, calcium and combinations thereof.
12. The pre-packaged bisulfite solution of claim 11 , wherein the counter-ion comprises magnesium.
13. The pre-packaged bisulfite solution of claim 11 , wherein the counter-ion comprises manganese.
14. The pre-packaged bisulfite solution of claim 11 , wherein the counter-ion comprises calcium.
15. The pre-packaged bisulfite solution according to claim 1 wherein the oxygen-impermeable container is selected from an ampoule, a hermetically sealed bag, and a capsule.
16. The pre-packaged bisulfite solution of claim 15 , wherein the oxygen-impermeable container comprises an ampoule.
17. The pre-packaged bisulfite solution of claim 16 , wherein the ampoule comprises an amber glass ampoule.
18. The pre-packaged bisulfite solution of claim 15 , wherein the oxygen-impermeable container comprises a capsule.
19. The pre-packaged bisulfite solution of claim 18 , wherein the capsule comprises a substantially oxygen-impermeable thermal plastic polymer.
20. The pre-packaged bisulfite solution according to claim 1 , wherein the bisulfite solution contains less than 10 ppm dissolved oxygen.