Method of inducing fenestrae
The present invention relates to a method of inducing fenestrae in an endothelial cell line. More particularly, the present invention relates to inducing fenestrae in a bEND5 cell line or a Py4.1 cell line utilizing latrunculin A or cytochalasin B as an inducing agent.
1 . A method of inducing the formation of fenestrae in an endothelial cell line selected from the group consisting of a bEND5 endothelial cell line and a Py4.1 endothelial cell line comprising the step of administering an inducing agent selected from the group consisting of latrunculin A and cytochalasin B.
2 . The method of claim 1 , wherein the endothelial cell line is a bEND5 endothelial cell line.
3 . The method of claim 1 , wherein the endothelial cell line is a Py4.1 endothelial cell line.
4 . The method of claim 1 , wherein the inducing agent is latrunculin A.
5 . The method of claim 4 , wherein latrunculin A is administered at a concentration of about 2.5 nM.
6 . The method of claim 1 , wherein the inducing agent is cytochalasin B.
7 . The method of claim 6 , wherein cytochalasin B is administered at a concentration of about 10 nM.
8 . The method of claim 1 , wherein the inducing agent induces the formation of fenestrae to greater than or equal to 100 fenestrae per cell.
9 . The method of claim 1 , wherein the inducing agent induces the formation of fenestrae to greater than or equal to 1000 fenestrae per cell.
10 . The method of claim 1 , wherein the inducing agent induces the formation of fenestrae greater than or equal to 10-fold from an un-induced endothelial cell line.
11 . The method of claim 1 , wherein the inducing agent induces the formation of fenestrae greater than or equal to 20-fold from an un-induced endothelial cell line.
12 . The method of claim 1 , wherein the inducing agent induces the formation of fenestrae greater than or equal to 50-fold from an un-induced endothelial cell line.
13 . The method of claim 1 , wherein the inducing agent induces the formation of fenestrae greater than or equal to 100-fold from an un-induced endothelial cell line.
14 . The method of claim 1 , wherein the inducing agent induces the formation of fenestrae greater than or equal to 200-fold from an un-induced endothelial cell line.
15 . The method of claim 1 , wherein the inducing agent induces the formation of fenestrae to greater than or equal to 3 fenestrae per μm 2 .
16 . The method of claim 1 , wherein the inducing agent induces the formation of fenestrae to greater than or equal to 5 fenestrae per μm 2 .
17 . The method of claim 1 , wherein the inducing agent induces the formation of fenestrae to greater than or equal to 60 fenestrae per μm 2 .
18 . The method of claim 1 , wherein the inducing agent induces the formation of fenestrae to about 3.5 fenestrae per μm 2 .
19 . The method of claim 1 , wherein the inducing agent induces the formation of fenestrae to about 4.5 fenestrae per μm 2 .
20 . The method of claim 1 , wherein the inducing agent induces the formation of fenestrae to about 5.3 fenestrae per μm 2 .
21 . A method of inducing the formation of fenestrae comprising the steps of administering latrunculin A to a bEND5 cell line.
22 . A method of inducing the formation of fenestrae comprising the step of administering latrunculin A to a Py4.1 cell line.
23 . A method of inducing the formation of fenestrae comprising the steps of administering cytochalasin B to a bEND5 cell line.
24 . A method of inducing the formation of fenestrae comprising the step of administering cytochalasin B to a Py4.1 cell line.
25 . A method of inducing the formation of fenestrae in a bEND5 cell line comprising the steps of:
a) administering cytochalasin B to the bEND5 cell line; and
b) administering VEGF-A to the bEND5 cell line.
26 . A method of inducing the formation of fenestrae in a bEND5 cell line comprising the steps of:
a) administering cytochalasin B to the bEND5 cell line; and
b) administering PMA to the bEND5 cell line.