Methods to produce lung surfactant formulations via lyophilization and formulations and uses thereof
Methods of producing lung surfactant formulations through solvent dissolution and lyophilization are described as well as surfactant formulations derived therefrom. Methods of treating respiratory distress dysfunction are also provided.
1. A method for producing a lyophilized lung surfactant composition having reduced viscosity upon reconstitution as compared to the viscosity of an identical liquid lung surfactant composition which was not lyophilized, the method comprising:
providing a lung surfactant polypeptide having at least 10 amino acid residues and represented by a formula:
(Z a U b ) c Z d
wherein Z represents a hydrophilic amino acid residue and U represents a hydrophobic amino acid residue; wherein each Z is independently R, D, E or K; and each U is independently V, I, L, C, Y, or F; and wherein a is an integer with an average value of about 1 to about 5; b is an integer with an average value of about 3 to about 20; c is an integer of about 1 to about 10; and d is an integer of about 1 to about 3;
combining the lung surfactant polypeptide, one or more phospholipids, and at least one or more of a fatty acid, a fatty alcohol or a fatty acid ester in an organic solvent system, said system comprising an organic solvent in an amount sufficient to (i) solubilize the lung surfactant polypeptide and lipids to form a substantially homogeneous liquid mixture, and (ii) reduce viscosity of the lyophilized lung surfactant upon reconstitution, wherein the organic solvent system comprises from about 30% by volume to about 70% by volume of the organic solvent with the remainder being aqueous solvent and the organic solvent comprises a lower oxyhydrocarbon; and
lyophilizing the substantially homogeneous liquid mixture to obtain the lyophilized lung surfactant composition having reduced viscosity upon reconstitution.
2. The method of claim 1 wherein the substantially homogeneous liquid mixture is filtered before lyophilization.
3. The method of claim 1 wherein the substantially homogeneous liquid mixture is filter sterilized before lyophilization.
4. The method of claim 1 wherein the lung surfactant polypeptide composition is sterilized after lyophilization.
5. The method of claim 1 wherein the lung surfactant polypeptide comprises SEQ ID NO:1 (KL4).
6. The method of claim 1 wherein the one or more phospholipids are selected from the group consisting of dipalmitoyl phosphatidylcholine (DPPC) and palmitoyloleoyl phosphatidylglycerol (POPG), and the fatty acid is palmitic acid.
7. The method of claim 1 wherein the organic solvent system comprises from about 40% by volume to about 60% by volume of organic solvent.
8. The method of claim 7 wherein the organic solvent system comprises about 50% by volume of organic solvent.
9. The method of claim 1 wherein the oxyhydrocarbon is methanol, ethanol, n-propanol, iso-propanol, n-butanol, iso-butanol, 2-butanol, t-butanol, pentanol, iso-pentanol, 2-pentanol, 3-pentanol, t-pentanol, methylethylketone, benzylalcohol, acetic acid, methylethyl ketone, or a combination thereof.
10. The method of claim 1 wherein the oxyhydrocarbon is methanol, ethanol, n-propanol, iso-propanol, n-butanol, iso-butanol, 2-butanol, t-butanol, or a combination thereof.
11. The method of claim 10 wherein the oxyhydrocarbon is t-butanol.
12. A method for producing a reconstituted liquid lung surfactant composition having reduced viscosity upon reconstitution as compared to an identical liquid lung surfactant composition which was not lyophilized, the method comprising:
(a) providing a lung surfactant polypeptide having at least 10 amino acid residues and represented by a formula:
(Z a U b ) c Z d
wherein Z represents a hydrophilic amino acid residue and U represents a hydrophobic amino acid residue; wherein each Z is independently R, D, E or K; and each U is independently V, I, L, C, Y, or F; and wherein a is an integer with an average value of about 1 to about 5; b is an integer with an average value of about 3 to about 20; c is an integer of about 1 to about 10; and d is an integer of about 1 to about 3;
(b) combining the lung surfactant polypeptide, one or more phospholipids and at least one or more of a fatty acid, a fatty alcohol or a fatty acid ester in an organic solvent system, said system comprising an organic solvent in an amount sufficient to (i) solubilize the lung surfactant polypeptide and lipids to form a substantially homogeneous liquid mixture and (ii) reduce viscosity of the lyophilized lung surfactant upon reconstitution provided that the organic solvent system comprises from about 30% by volume to about 70% by volume of the organic solvent with the remainder being aqueous solvent and the organic solvent comprises a lower oxyhydrocarbon;
(c) lyophilizing the substantially homogeneous liquid mixture to obtain the lyophilized lung surfactant composition; and
(d) reconstituting the lyophilized lung surfactant composition with a sufficient amount of a pharmaceutically acceptable dispersing agent to yield a reconstituted liquid lung surfactant composition, wherein the reconstituted liquid lung surfactant composition has reduced viscosity as compared to the viscosity of an identical liquid lung surfactant composition which was not lyophilized.
13. The method of claim 12 wherein the reconstituted liquid lung surfactant composition comprises SEQ. ID. NO: 1 (KL4).