Method to dry microtitration filter tray cavities and received filters therein
View Patent ↗An apparatus for drying microtitration filter tray cavities and filters configured therein using a flow of gas, the cavities each comprising an upper and a lower aperture and the microtitration filter tray being mounted on a chamber designed for the vacuum filtration of microtitration filter trays in a manner that a vacuum can be applied to the lower apertures of the cavities receiving the filters, and includes a heat exchanger which can be mounted on and later removed from the microtitration filter tray and can be heated and comprises at least one inlet aperture and at least one outlet aperture between which a flow of gas may move through the heat exchanger, the heat exchanger being designed to heat the gas flow and to guide it onto the upper cavity apertures, the gas flow being generated by a vacuum pump communicating with the vacuum chamber.
1. A method for handling a microtitration tray ( 13 ) with cavities ( 12 ) each comprising an upper aperture and a lower aperture ( 17 ) receiving filters ( 11 ) within the cavities ( 12 ), comprising the steps of:
(1) configuring the microtitration filter tray ( 13 ) on a chamber ( 14 ) designed for vacuum filtration of microtitration filter trays ( 13 );
(2) pipetting samples into the individual microfiltration filter tray cavities ( 12 );
(3) aspirating the samples through the filters ( 11 ) by applying a vacuum to the lower apertures ( 17 ) of the cavities ( 12 ) receiving the filters ( 11 );
(4) applying several times a washing solution into the cavities ( 12 ) and aspirating the solution through the filters ( 11 ) and/or using an elution buffer to remove significant components of the samples from the filters ( 11 );
(5) mounting a heat exchanger ( 21 ) detachably on the microtitration filter tray ( 13 ) which is either heated before being mounted or is heated after being mounted, said heat exchanger ( 21 ) comprising at least one inlet aperture and at least one outlet aperture ( 22 ) allowing a gas flow ( 23 ) to move between them; and
(6) generating a gas flow to pass through the filters ( 11 ) by a vacuum pump ( 16 ) communicating with the vacuum chamber ( 14 ) using said heat exchanger ( 21 ) to heat the gas flow ( 23 ) and guide the gas flow to the upper apertures of the cavities ( 12 ), the gas flow ( 23 ) moving from the inlet aperture to the outlet aperture of said heat exchanger ( 21 ).
2. The method as claimed in claim 1 , further including the step of displacing the heat exchanger ( 21 ) between a heating position ( 34 ) and the position on the microtitration filter tray ( 13 ), in automated manner by an associated transporter ( 31 , 32 ).
3. The method as claimed in claim 2 , wherein the heat exchanger ( 21 ) is heated passively and when in a heating position ( 34 ) makes contact over a large area with a thermostatting unit ( 33 ).