Granted Patent B2

US 7,476,734 · App. 11/295,155 · Granted Jan 13, 2009

Abstract

Claims (13)

1. A nucleotide analog having the structure:

wherein X 1 is OH or PO 4 ; X 2 is H or OH; B is selected from the group consisting of a purine, a pyrimidine and derivatives thereof; CH═CHNHC(O)OC 2 H 4 NHCH 2 SO 2 is a linker; and X 3 is O or S.

2. The nucleotide analog of claim 1 , wherein said label is an optically-detectable label.

3. The nucleotide analog of claim 2 , wherein said optically-detectable label is a fluorescent label.

4. The nucleotide analog of claim 1 , wherein B is selected from the group consisting of cytosine, uracil, thymine, adenine, guanine, and analogs thereof.

5. The nucleotide analog of claim 1 , wherein said linker comprises a cleavable bond.

6. The nucleotide analog of claim 5 , wherein said cleavable bond is a chemically cleavable bond.

7. The nucleotide analog of claim 5 , wherein said cleavable bond is a photochemically cleavable bond.

8. The nucleotide analog of claim 5 , wherein said cleavable bond is cleaved upon exposure to a pH from about 11.3 to about 12.3.

9. The nucleotide analog of claim 5 , wherein said cleavable bond is cleaved upon exposure to a pH of about 11.8.

10. A nucleotide analog having the structure:

11. The nucleotide analog of claim 10 , wherein said label is an optically-detectable label.

12. The nucleotide analog of claim 11 , wherein said optically-detectable label is a fluorescent label.

Assignments (8)