Methods of producing polyclonal antibodies
The invention includes transgenic avians which produce eggs containing human polyclonal antibody and methods of making such avians.
1 . A transgenic avian which produces eggs containing human polyclonal antibody.
2 . The transgenic avian of claim 1 wherein the avian is a chicken.
3 . The transgenic avian of claim 1 wherein the avian is a G1 transgenic avian.
4 . The transgenic avian of claim 1 wherein the avian is a G2 transgenic avian.
5 . The transgenic avian of claim 1 wherein the avian is selected from the group consisting of chicken, quail and turkey.
6 . The transgenic avian of claim 1 wherein a cell of the avian comprises an artificial chromosome containing coding sequences for the polyclonal antibody.
7 . The transgenic avian of claim 6 wherein the artificial chromosome comprises a centromere selected from the group consisting of an insect centromere, a mammalian centromere and an avian centromere.
8 . The transgenic avian of claim 1 comprising a human Ig locus in its germline.
9 . The transgenic avian of claim 1 comprising one or more of Igλ, Igκ, IgH, portions thereof or combinations thereof in its germline.
10 . The transgenic avian of claim 1 wherein the polyclonal antibody is present in an egg laid by the transgenic avian in an amount in a range of about 10 ng to about 1 gram.
11 . The transgenic avian of claim 1 wherein the polyclonal antibody is present in an egg laid by the transgenic avian in an amount in a range of about 10 μg to about 1 gram.
12 . A transgenic avian which produces eggs comprising human polyclonal antibody wherein the avian contains an artificial chromosome which comprises an Ig loci.
13 . The transgenic avian of claim 12 wherein the artificial chromosome is a satellite artificial chromosome.
14 . The transgenic avian of claim 12 comprising an Ig loci in its germline.
15 . The transgenic avian of claim 12 comprising one or more of Igλ, Igκ, IgH, portions thereof or combinations thereof in its germline.
16 . An avian egg containing a human polyclonal antibody.
17 . The egg of claim 16 wherein the polyclonal antibody is present in an amount in a range of about 10μ to about 1 gram.
18 . The egg of claim 16 wherein the polyclonal antibody is present in an amount in a range of about 50μ to about 1 gram.
19 . A method comprising,
producing an artificial chromosome in a cell wherein a transgene is introduced into the artificial chromosome during assembly of the artificial chromosome.
20 . The method of claim 19 wherein the transgene comprises at least one of a promoter and a coding sequence.
21 . The method of claim 21 wherein the transgene is of a size in a range of between about 8 kb and about 100 mb.
22 . The method of claim 19 wherein the transgene comprises at least one Ig gene.
23 . The method of claim 22 wherein an Ig gene is a human Ig gene.
24 . The method of claim 19 wherein the transgene comprises at least one member selected from the group consisting of an Igλ gene, an Ig H gene and an IgK gene.
25 . A transgenic avian comprising an artificial chromosome wherein the artificial chromosome contains at least one Ig gene.
26 . The transgenic avian of claim 25 wherein the at least one Ig gene is selected from the group consisting of an Igλ gene, an Ig H gene and/an IgK gene.
27 . The transgenic avian of claim 25 wherein the transgenic avian is a G1 transgenic avian.