Bone delivery device
View Patent ↗Bone cages are disclosed including devices for biocompatible implantation. The structures of bone are useful for providing living cells and tissues as well as biologically active molecules to subjects.
1. A method of making a device for implantation in a subject, comprising:
micromachining hard bone tissue that includes a natural internal cavity therein to form an implantable structure exhibiting at least one dimension of about 30 μm to about 90 μm;
micromachining the implantable structure to re-shape the natural internal cavity thereof;
micromachining a plurality of holes in the implantable structure that access the natural internal cavity; and
covering at least a portion of the plurality of holes of the implantable structure with a semi-permeable component comprising a layer of confluent cells prior to implantation of the device in the subject.
2. The method of claim 1 , wherein micromachining the implantable structure to re-shape the internal cavity thereof is by focused beam machining the implantable structure.
3. The method of claim 1 , wherein the covering is by placing the implantable structure in the semi-permeable component.
4. The method of claim 1 , wherein the covering is by placing the semi-permeable component on the interior surface of the cavity in the implantable structure.
5. The method of claim 1 , further comprising:
identifying one or more biologically active molecules; and
providing the one or more biologically active molecules to the micromachined internal cavity.
6. The method of claim 1 , further comprising:
identifying one or more living cells or tissues; and
providing the one or more living cells or tissues to the micromachined internal cavity.
7. The method of claim 1 , wherein the bone is organic.
8. The method of claim 1 , wherein the bone is anorganic.
9. The method of claim 1 , wherein the hard bone tissue is demineralized.
10. The method of claim 1 , wherein the hard bone tissue is freeze-dried.
11. The method of claim 1 , wherein the hard bone tissue is autologous with respect to the subject.
12. The method of claim 1 , wherein the hard bone tissue is allogeneic with respect to the subject.
13. The method of claim 1 , wherein the hard bone tissue is xenogeneic with respect to the subject.
14. The method of claim 1 , wherein the hard bone tissue is immunogenic with respect to the subject.
15. The method of claim 1 , wherein the hard bone tissue is non-immunogenic with respect to the subject.
16. The method of claim 1 , further comprising: treating the hard bone tissue to at least partially prevent restructuring.
17. The method of claim 1 , further comprising: treating the hard bone tissue to enhance restructuring.
18. The method of claim 1 , further comprising;
surrounding the implantable structure with the semi-permeable component.
19. The method of claim 1 , wherein the implantable structure surrounds the semi-permeable component.
20. A method of making a device for implantation in a subject, comprising:
micromachining hard bone tissue to form an implantable structure exhibiting at least one dimension of about 30 μm to about 90 μm;
forming an internal cavity in the implantable structure;
forming a plurality of holes in the implantable structure that access the internal cavity; and
covering at least a portion of the plurality of holes with a semi-permeable component comprising a layer of confluent cells prior to implantation of the device in the subject.
21. The method of claim 20 , wherein covering at least a portion of the plurality of holes with a semi-permeable component comprising a layer of confluent cells includes covering at least a portion of an exterior of the implantable structure with the layer of confluent cells.
22. The method of claim 20 , wherein covering at least a portion of the plurality of holes with a semi-permeable component comprising a layer of confluent cells includes covering at least a portion of an interior of the implantable structure with the layer of confluent cells.