Ion fragmentation parameter selection systems and methods
View Patent ↗In some embodiments, a tandem (MS/MS) mass spectrometry method includes selecting a collision-induced dissociation (CID) voltage amplitude and a q-parameter value for a quadrupole ion trap to optimize a daughter ion fragmentation process for a given parent ion mass-to-charge (m/z) ratio. The q and CID voltage values may be selected according to a look-up table and/or using approximate analytical expressions. The correspondence between m/z values and (q, CID) value pairs may be established by pre-measurement calibration. A fragmentation-optimized q value may be computed according to m/z, and a CID voltage value may be determined according to the computed q value. A user may also force q to another value, for example in order to facilitate trapping of a desired daughter ion mass range, and the controller computes a CID voltage value according to the forced q value.
1. A mass spectrometry method comprising:
selecting a collision-induced-dissociation (CID) voltage amplitude according to a q parameter value for a parent ion; and
inducing a fragmentation of the parent ion by applying a CID voltage having the CID voltage amplitude to an ion trap holding the parent ion.
2. The method of claim 1 , further comprising trapping the parent ion according to the q parameter value while inducing the fragmentation of the parent ion.
3. The method of claim 1 , comprising selecting the CID voltage amplitude according to a mass-to-charge ratio of the parent ion.
4. The method of claim 1 , further comprising selecting the q parameter value according to a mass-to-charge ratio of the parent ion to optimize an efficiency of the fragmentation of the parent ion.
5. The method of claim 1 , further comprising receiving a user selection of the q parameter value.
6. The method of claim 1 , wherein selecting the CID voltage amplitude comprises employing a table establishing a correspondence between parent ion mass-to-charge ratios, corresponding fragmentation-optimized q parameter values and CID voltage amplitudes.
7. The method of claim 1 , wherein selecting the CID voltage amplitude comprises employing an analytical expression establishing a dependence of the CID voltage amplitude on the q parameter value and a mass-to-charge ratio of the parent ion.
8. The method of claim 1 , further comprising performing a set of calibration measurements establishing a dependence of a set of fragmentation-optimized q parameter values and CID voltage amplitudes on a set of corresponding parent ion mass-to-charge ratios.
9. The method of claim 1 , further comprising detecting a daughter ion resulting from the fragmentation of the parent ion.
10. A mass spectrometry method comprising:
from a mass-to-charge ratio of a parent ion, determining a q parameter value and a collision-induced dissociation (CID) voltage amplitude to optimize an efficiency of a fragmentation of the parent ion; and
inducing the fragmentation of the parent ion according to the q parameter value and the CID voltage amplitude.
11. A mass spectrometry method comprising:
selecting a collision-induced-dissociation (CID) voltage amplitude for a parent ion according to a mass-to-charge ratio of the parent ion and an ion trap drive voltage indicator; and
inducing a fragmentation of the parent ion in the ion trap according to the CID voltage amplitude and the ion trap drive voltage indicator.
12. The method of claim 11 , wherein the ion trap drive voltage indicator comprises a q parameter value.
13. The method of claim 11 , wherein the ion trap drive voltage indicator comprises a drive voltage amplitude.
14. A mass spectrometry apparatus comprising:
a quadrupole ion trap including a central ring electrode and a pair of endcap electrodes disposed on opposite sides of the central ring electrode; and
a mass spectrometer controller connected to the ion trap and configured to:
select a collision-induced-dissociation (CID) voltage amplitude according to a q parameter value for a parent ion and a mass-to-charge ratio of the parent ion;
trap the parent ion in the ion trap by applying a drive voltage according to the q parameter value to the central ring electrode; and
while trapping the parent ion, induce a fragmentation of the parent ion by applying a CID voltage having the CID voltage amplitude across the endcap electrodes.
15. The apparatus of claim 14 , wherein the controller is configured to select the q parameter value according to the mass-to-charge ratio of the parent ion to optimize an efficiency of the fragmentation of the parent ion.
16. The apparatus of claim 14 , wherein the controller is configured to receive a user selection of the q parameter value.
17. The apparatus of claim 14 , wherein the controller is configured to selecting the CID voltage amplitude by employing a table establishing a correspondence between parent ion mass-to-charge ratios, corresponding fragmentation-optimized q parameter values and CID voltage amplitudes.
18. The apparatus of claim 14 , wherein the controller is configured to select the CID voltage amplitude by employing an analytical expression establishing a dependence of the CID voltage amplitude on the q parameter value and the mass-to-charge ratio of the parent ion.
19. The apparatus of claim 14 , wherein the controller is configured to perform a set of calibration measurements establishing a dependence of a set of fragmentation-optimized q parameter values and CID voltage amplitudes on a set of corresponding parent ion mass-to-charge ratios.
20. The apparatus of claim 14 , further comprising a detector connected to the ion trap and configured to detect a daughter ion resulting from the fragmentation of the parent ion.