Maize Cyclo1 gene and promoter
View Patent ↗The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a root-preferred promoter for the gene encoding Cyclo1. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stabling incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence. The present invention also relates to isolated nucleic acids encoding plant cyclotides. The invention relates to the construction of a chimeric gene encoding all or a portion of the plant cyclotides, in sense or antisense orientation, wherein expression of the chimeric gene results in the production of altered levels of plant cyclotides in a transformed host cell.
1. An isolated nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of:
a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:1 or a full-length complement thereof;
b) a nucleotide sequence comprising the plant promoter sequence of the plasmid deposited as Patent Deposit No: NRRL B-30794, or a full-length complement thereof; and
c) a nucleotide sequence comprising a fragment of the sequence set forth in SEQ ID NO:1, wherein said sequence initiates transcription in a plant cell.
2. A DNA construct comprising the nucleic acid of claim 1 operably linked to a heterologous nucleotide sequence of interest.
3. A vector comprising the DNA construct of claim 2 .
4. A plant cell having stably incorporated into its genome the DNA construct of claim 2 .
5. The plant cell of claim 4 , wherein said plant cell is from a monocot.
6. The plant cell of claim 5 , wherein said monocot is maize.
7. The plant cell of claim 4 , wherein said plant cell is from a dicot.
8. A plant having stably incorporated into its genome the DNA construct of claim 2 .
9. The plant of claim 8 , wherein said plant is a monocot.
10. The plant of claim 9 , wherein said monocot is maize.
11. The plant of claim 8 , wherein said plant is a dicot.
12. A transgenic seed of the plant of claim 8 , wherein the seed comprises the DNA construct.
13. The plant of claim 8 , wherein the heterologous nucleotide sequence of interest encodes a gene product that confers herbicide, salt, cold, drought, pathogen, or insect resistance.
14. A method for expressing a nucleotide sequence in a plant, said method comprising introducing into a plant a DNA construct, said DNA construct comprising a promoter and operably linked to said promoter a heterologous nucleotide sequence of interest, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:1;
b) a nucleotide sequence comprising the plant promoter sequence of the plasmid designated as Patent Deposit No: NRRL B-30794; and
c) a nucleotide sequence comprising a fragment of the sequence set forth in SEQ ID NO:1, wherein said nucleotide sequence initiates transcription in said plant.
15. The method of claim 14 , wherein said plant is a dicot.
16. The method of claim 14 , wherein said plant is a monocot.
17. The method of claim 16 , wherein said monocot is maize.
18. The method of claim 14 , wherein the heterologous nucleotide sequence encodes a gene product that confers herbicide, salt, cold, drought, pathogen, or insect resistance.
19. The method of claim 14 , wherein said heterologous nucleotide sequence of interest is selectively expressed in the root.
20. A method for expressing a nucleotide sequence in a plant cell, said method comprising introducing into a plant cell a DNA construct comprising a promoter operably linked to a heterologous nucleotide sequence of interest, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:1;
b) a nucleotide sequence comprising the plant promoter sequence of the plasmid designated as Patent Deposit No: NRRL B-30794; and
c) a nucleotide sequence comprising a fragment of the sequence set forth in SEQ ID NO:1, wherein said nucleotide sequence initiates transcription in said plant cell.
21. The method of claim 20 , wherein said plant cell is from a monocot.
22. The method of claim 21 , wherein said monocot is maize.
23. The method of claim 20 , wherein said plant cell is from a dicot.
24. The method of claim 20 , wherein the heterologous nucleotide sequence encodes a gene product that confers herbicide, salt, cold, drought, pathogen, or insect resistance.
25. A method for selectively expressing a nucleotide sequence in a plant root, said method comprising introducing into a plant cell a DNA construct, and regenerating a transformed plant from said plant cell, said DNA construct comprising a promoter and a heterologous nucleotide sequence operably linked to said promoter, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:1;
b) a nucleotide sequence comprising the plant promoter sequence of the plasmid deposited as Patent Deposit No: NRRL B-30794; and
c) a nucleotide sequence comprising a fragment of the sequence set forth in SEQ ID NO:1, wherein said sequence initiates transcription in a plant root cell.
26. The method of claim 25 , wherein the plant is a monocot.
27. The method of claim 26 , wherein the monocot is maize.
28. The method of claim 25 , wherein the plant is a dicot.
29. The method of claim 25 , wherein the heterologous nucleotide sequence encodes a gene product that confers herbicide, salt, pathogen, or insect resistance.