Bone marrow stromal cells for immunoprotection of transplanted neural stem cells
The present invention encompasses methods and compositions for reducing an immune response to a transplant recipient receiving NSCs by treating said recipient with an amount of bone marrow stromal effective to reduce or inhibit host rejection of the NSCs.
1 . A method of treating a transplant recipient, wherein said transplant is a neural stem cell (NSC), to reduce in said recipient an immune response of effector cells against an alloantigen to said effector cells, the method comprising;
administering to a transplant recipient, bone marrow stromal cells in an amount effective to reduce an immune response of effector cells against an alloantigen to said effector cells, whereby in the transplant recipient said effector cells have a reduced immune response against said alloantigen.
2 . The method of claim 1 , wherein said effector cells are T cells.
3 . The method of claim 2 , wherein said T cells are from a donor and said alloantigen is from a recipient.
4 . The method of claim 2 , wherein said T cells are from a recipient and said alloantigen is from a donor.
5 . The method of claim 2 , wherein said T cells are present in the transplant.
6 . The method of claim 1 , wherein prior to said administering to a transplant recipient bone marrow stromal cells, said bone marrow stromal cells have been expanded in culture.
7 . The method of claim 1 , wherein said effector cells are T cells activated prior to administration of bone marrow stromal cells, and further wherein said immune response is the reactivation of said T cells from the donor.
8 . The method of claim 1 , wherein said bone marrow stromal cells are administered to the transplant recipient to treat rejection of the transplant by the recipient.
9 . The method of claim 1 , wherein said bone marrow stromal cells are human bone marrow stromal cells.
10 . The method of claim 1 , wherein said bone marrow stromal cells are rat bone marrow stromal cells.
11 . The method of claim 1 , wherein said NSCs are human NSCs.
12 . The method of claim 1 , further comprising administering to said recipient an immunosuppressive agent.
13 . The method of claim 1 , wherein said bone marrow stromal cells are administered to the recipient prior to said transplant.
14 . The method of claim 1 , wherein said bone marrow stromal stem cells are administered to the recipient concurrently with said transplant.
15 . The method of claim 14 , wherein said bone marrow stromal cells are administered as part of the transplant.
16 . The method of claim 1 , wherein said bone marrow stromal cells are administered to the recipient subsequent to the transplantation of the transplant.
17 . The method of claim 1 , wherein said bone marrow stromal cells are administered intravenously to the recipient.
18 . The method of claim 1 , wherein said effector cells are cells of a recipient of said donor transplant.
19 . The method of claim 1 , wherein said bone stromal cells are genetically modified.
20 . A method for treating a transplant recipient, wherein said transplant is a neural stem cell (NSC), to reduce in said recipient an immune response of effector cells against an alloantigen to the effector cells, comprising:
transplanting to a transplant recipient NSCs with bone marrow stromal cells in an amount effective to reduce an immune response of effector cells against an alloantigen to the effector cells, whereby in the transplant recipient the effector cells have a reduced immune response against the alloantigen.
21 . The method of claim 20 wherein said effector cells are T cells.