Methods and compositions for thickening, stabilizing and emulsifying foods
Provided herein are novel food additive polysaccharides. The polysaccharides of the invention can be used, for example to emulsify a food or to suspend compounds in a food composition. Also provided are algae capable of incorporating compounds into polysaccharides to alter the rheological properties of the polysaccharides. Also provided are large scale, low cost methods of precipitating and purifying novel hydrocolloids.
1 - 45 . (canceled)
46 . The method of claim 69 , wherein the cell is of a species listed in Table 1.
47 - 68 . (canceled)
69 . A method of trophically converting a cell of the genus Porphyridium comprising:
a. transforming said cell with a nucleic acid encoding a carbohydrate transporter protein; and
b. selecting for the ability to undergo cell division in the presence of fixed carbon material as the sole energy source for the cell, wherein the fixed carbon material is a carbohydrate that is transported by the carbohydrate transporter protein.
70 . The method of claim 69 , wherein the carbohydrate transporter protein has at least 60% amino acid identity with one or more of SEQ ID NOs: 12, 14, 16, 18 and 19.
71 . The method of claim 69 , wherein the nucleic acid encoding a carbohydrate transporter protein is in operable linkage with a promoter active in microalgae.
72 . The method of claim 69 , wherein the carbohydrate is selected from Tables 2 or 3.
73 . The method of claim 69 , wherein the nucleic acid also comprises a segment encoding an additional protein.
74 . The method of claim 73 , wherein the additional protein is selected from the group consisting of an enzyme that produces small molecules, a mammalian growth hormone, and a protein that confers resistance to an antibiotic.
75 . The method of claim 74 , wherein the additional protein confers resistance to the antibiotic zeocin.
76 . The method of claim 73 , wherein the segment encoding an additional protein and the nucleic acid encoding a carbohydrate transporter are each in operable linkage with a promoter which directs their expression in said cell.
77 . The method of claim 69 , wherein the nucleic acid encoding a carbohydrate transporter segment utilizes preferred codons of the cell.
78 . The method of claim 69 , wherein the carbohydrate that is transported by the carbohydrate transporter protein is selected from the group consisting of D-galactose, α-D-glucose, α-D-Glucose-1-Phosphate, Glucose, D-Glucose-6-Phosphate, and floridoside.
79 . The method of claim 75 , further comprising the step of selecting for the ability to survive in the presence of zeocin.