Coccidial vaccine and methods of making and using same
View Patent ↗The present invention relates to a vaccine for coccidiosis in chickens prepared from three attenuated Eimeria species: E. acervulina, E. maxima and E. tenella . The vaccine was similar to or superior to other anticoccidial drugs in stimulating protective immunity against coccidiosis.
1. An immunogenic or vaccine composition for protection against E. acervulina, E. maxima and E. tenella consisting of a pharmaceutically acceptable excipient and a mixture of sporulated oocysts isolated from precocious strains of E. acervulina, E. maxima and E. tenella , wherein the mixture is about 500 oocysts of E. acervulina , about 50 to about 100 oocysts of E. maxima and about 100 to about 250 oocysts of E. tenella.
2. The composition of claim 1 wherein the mixture is about 500 oocysts of E. acervulina , about 100 oocysts of E. maxima and about 100 oocysts of E. tenella.
3. The composition of claim 1 wherein the mixture is about 500 oocysts of E. acervulina , about 50 oocysts of E. maxima and about 250 oocysts of E. tenella.
4. An immunogenic or vaccine composition for protection against E. acervulina, E. maxima and E. tenella consisting of a pharmaceutically acceptable excipient and a mixture of sporulated oocysts, wherein the mixture of sporulated oocytes consists of sporulated oocysts isolated from precocious strains of E. acervulina, E. maxima and E. tenella , wherein for every 10 sporocysts of E. acervulina , there are about 1 to 2 sporocysts of E. maxima and about 2 to 10 sporocysts of E. tenella.
5. The composition of claim 4 wherein for every 10 sporocysts of E. acervulina , there are about 2 sporocysts of E. maxima and about 2 sporocysts of E. tenella .
6. A method of eliciting an immune response comprising administering an effective amount of the immunogenic or vaccine composition of claim 1 to induce the response in a chicken.
7. A method for inducing an immunological or protective response comprising administering an effective amount of the immunogenic or vaccine composition of claim 1 to induce the response in a chicken.
8. A method of eliciting an immune response comprising administering an effective amount of the immunogenic or vaccine composition of claim 4 to induce the response in a chicken.
9. A method for inducing an immunological or protective response comprising administering an effective amount of the immunogenic or vaccine composition of claim 4 to induce the response in a chicken.
10. The method of claim 6 wherein the effective amount is about 500 oocysts of E. acervulina , about 50 to about 100 oocysts of E. maxima and about 100 to about 250 oocysts of E. tenella.
11. The method of claim 7 wherein the effective amount is about 500 oocysts of E. acervulina , about 50 to about 100 oocysts of E. maxima and about 100 to about 250 oocysts of E. tenella.
12. The method of claim 6 wherein the effective amount is about 500 oocysts of E. acervulina , about 100 oocysts of E. maxima and about 100 oocysts of E. tenella.
13. The method of claim 7 wherein the effective amount is about 500 oocysts of E. acervulina , about 100 oocysts of E. maxima and about 100 oocysts of E. tenella.
14. The method of claim 8 wherein the effective amount of the mixture of sporulated oocysts, wherein for every 10 sporocysts of E. acervulina , there are about 2 sporocysts of E. maxima and about 2 sporocysts of E. tenella in the mixture.
15. The method of claim 9 wherein the effective amount of the mixture of sporulated oocysts, wherein for every 10 sporocysts of E. acervulina , there are about 2 sporocysts of E. maxima and about 2 sporocysts of E. tenella in the mixture.
16. The method of claim 6 wherein the effective amount is sufficient to resist a challenge dose of about 100,000 to about 500,000 oocysts of E. acervulina and about 10,000 to about 1,000,000 oocysts of E. maxima or about 10,000 to about 100,000 oocysts of E. tenella to the animal.
17. The method of claim 7 wherein the effective amount is sufficient to resist a challenge dose of about 100,000 to about 500,000 oocysts of E. acervulina and about 10,000 to about 100,000 oocysts of E. maxima or about 10,000 to about 100,000 oocysts of E. tenella to the animal.
18. The method of claim 8 wherein the effective amount is sufficient to resist a challenge dose of about 100,000 to about 500,000 oocysts of E. acervulina and about 10,000 to about 1,000,000 oocysts of E. maxima or about 10,000 to about 100,000 oocysts of E. tenella to the animal.
19. The method of claim 9 wherein the effective amount is sufficient to resist a challenge dose of about 100,000 to about 500,000 oocysts of E. acervulina and about 10,000 to about 1,000,000 oocysts of E. maxima or about 10,000 to about 100,000 oocysts of E. tenella to the animal.