Centrifugal separation apparatus and method for separating fluid components
An apparatus and method are provided for separating components of a fluid or particles. A separation vessel having a barrier dam is provided to initially separate an intermediate density components of a fluid, and a fluid chamber is provided to further separate these intermediate density components by forming an elutriative field or saturated fluidized particle bed. The separation vessel includes a shield for limiting flow into the fluid chamber of relatively high density substances, such as red blood cells. The separation vessel also includes a trap dam with a smooth, gradually sloped downstream section for reducing mixing of substances. Structure is also provided for adding additional plasma to platelets and plasma flowing from the fluid chamber. The system reduces clumping of platelets by limiting contact between the platelets and walls of the separation vessel.
1. A method of reducing clumping of platelets during separation of blood components, comprising:
providing a separation vessel including an inlet portion, an outlet portion, and a flow path between the inlet and outlet portions;
rotating the separation vessel about an axis of rotation;
introducing blood components into the rotating separation vessel such that the blood components stratify in the separation vessel to form at least a radial outer layer including red blood cells, an intermediate layer including at least platelets, and a radial inner layer including low density substances;
removing the platelets from the separation vessel via an outlet port in the outlet portion;
maintaining the radially outer layer of red blood cells between the intermediate layer and a radially outer wall of the separation vessel to substantially limit contact between the platelets and the radially outer wall of the separation vessel and thereby reduce platelet clumping; and
maintaining the radial inner layer of low density substances between the intermediate layer and a radially inner wall of the separation vessel to substantially limit contact between the platelets and the radially inner wall of the separation vessel and thereby reduce platelet clumping,
wherein the maintaining of the radially inner layer of low density substances includes trapping the low density substances with a trap dam.
2. The method of claim 1 , wherein the low density substances include at least one of saline and plasma.
3. The method of claim 1 , wherein the radially outer layer of red blood cells is maintained along the radially outer wall of the separation vessel between the inlet and the outlet portions.
4. The method of claim 1 , wherein the layers cause increased platelet velocities and scrubbing of platelets toward the outlet port.
5. A method of reducing clumping of platelets during separation of blood components, comprising:
providing a separation vessel including an inlet portion, an outlet portion, and a flow path between the inlet and outlet portions;
rotating the separation vessel about an axis of rotation;
introducing blood components into the rotating separation vessel such that the blood components stratify in the separation vessel to form at least a radial outer layer including high density substances, an intermediate layer including at least platelets, and a radial inner layer including low density substances;
removing the platelets from the separation vessel via an outlet port in the outlet portion; and
maintaining the radially inner layer of low density substances between the intermediate layer and a radially inner wall of the separation vessel to substantially limit contact between the platelets and the radially inner wall of the separation vessel and thereby reduce platelet clumping,
wherein the maintaining of the radially inner layer of low density substances includes trapping the low density substances with a trap dam.
6. The method of claim 5 , wherein the low density substances include at least one of saline and plasma.
7. The method of claim 5 , wherein the radially inner layer of low density substances is maintained along the radially inner wall of the separation vessel.
8. The method of claim 5 , wherein the layers cause increased platelet velocities and scrubbing of platelets toward the outlet port.