Method and system removing fluorescence and other slowly varying baseline in Raman spectra
View Patent ↗A data set processing method for Raman spectroscopy systems using tunable lasers and multielement spectrometers compiles the spectral data set into an array and then estimates the background component, which is usually dominated by sample and optical train fluorescence, detector array dark current signal, fixed-pattern signal, and stray-light signals either modulated or non-modulated by in-path optics. This estimate is used as a baseline correction to the spectral data set to thereby isolate the sample's Raman response.
1. A method of processing spectral data from a Raman spectroscopy system, comprising:
a source for illuminating a sample at a plurality of wavelengths within a scan band; and
a spectrometer including an array of detection elements for detecting a spectral response of the sample in response to illumination by the source at the plurality of wavelengths;
wherein the method comprises:
compiling a spectral data set including the responses of the detection elements for each of the plurality of wavelengths;
characterizing the responses of the detection elements based on levels of change in the responses with changes in the wavelength of the illumination;
determining a baseline from the responses; and
correcting the spectral data set using the determined baseline.
2. A method as claimed in claim 1 , wherein the spectrometer of the Raman spectroscopy system further comprises a dispersive element of dispersing the spectral response over the array of detection elements.
3. A method as claimed in claim 1 , wherein the step of compiling the spectral data set comprises placing the spectral data set in an array of responses for each of the detection elements for each of the plurality of wavelengths.
4. A method as claimed in claim 1 , wherein the step of characterizing the responses comprises analyzing the responses of each detection elements for changes with changes in the wavelength of the illumination.
5. A method as claimed in claim 1 , wherein the step of characterizing the responses comprises analyzing the responses of each detection element based on the deviation in the responses with changes in the wavelength of the illumination.
6. A method as claimed in claim 1 , wherein the step of determining the baseline comprising determining a best fit polynomial.
7. A method as claimed in claim 1 , wherein the step of correcting the spectral data set comprises subtracting the baseline from the spectral data set.
8. A Raman spectroscopy system, comprising:
a source for illuminating a sample at a plurality of wavelengths within a scan band;
a spectrometer including an array of detection elements for detecting a spectral response of the sample in response to illumination by the source at the plurality of wavelengths; and
a system controller for controlling the source and receiving responses of the detection elements of the spectrometer, the system controller processing the spectral data to determine a Raman response of the sample by compiling a spectral data set including the responses of detection elements for each of the plurality of wavelengths, characterizing the responses of the detection elements based on levels of change in the responses with changes in the wavelength of the illumination, determining a baseline from the responses, and correcting the spectral data set in response to the determined baseline.
9. A system as claimed in claim 8 , wherein the spectrometer of the Raman spectroscopy system further comprises a dispersive element of dispersing the spectral response over the array of detection elements.
10. A system as claimed in claim 8 , wherein the source comprises a tunable laser.
11. A system as claimed in claim 8 , wherein the system controller compiles the spectral data set by placing the spectral data set in an array with responses for each of the detection elements for each of the plurality of wavelengths.
12. A system as claimed in claim 8 , wherein the system controller characterizes the responses by analyzing the responses of each detection element for changes with changes in the wavelength of the illumination.
13. A system as claimed in claim 8 , wherein the system controller characterizes the responses by analyzing the responses of each detection element based on the deviation in the responses with changes in the wavelength of the illumination.
14. A system as claimed in claim 8 , wherein the system controller determines the baseline by determining a best fit polynomial.
15. A system as claimed in claim 8 , wherein the step of correcting the spectral data set comprises subtracting the baseline from the spectral data set.