Implantable medical articles having laminin coatings and methods of use
Laminin-containing coatings for the surfaces of implantable medical devices are disclosed. The coatings promote the formation of vessels in association with the coated surfaces with minimal fibrotic resonse.
1 . A method for causing the formation of blood vessels in association with a coating on the surface of an implantable medical article comprising steps of:
implanting the medical article in a subject, the coating comprising laminin-5, an active portion thereof, or a binding member thereof; and
maintaining the medical article in the subject for a period of time sufficient to cause the formation of blood vessels in association with the coated surface.
2 . The method of claim 1 wherein the step of implanting comprises delivering the medical article to an intravascular location in the subject.
3 . The method of claim 1 wherein the step of maintaining results in the formation of a acellular fibrotic capsule of less than 100 mm in thickness on the implantable medical article.
4 . The method of claim 1 wherein the step of maintaining results in the formation of a acellular fibrotic capsule of less than 75 mm in thickness on the implantable medical article.
5 . A method for causing the formation of blood vessels in association with a coating on the surface of an implantable medical article comprising steps of:
implanting the medical article in a subject, the coating comprising
a laminin, an active portion thereof, or a binding member thereof, and
a collagen, an active portion thereof, or a binding member thereof,
maintaining the medical article in the subject for a period of time sufficient to cause the formation of blood vessels in association with the coated surface.
6 . An implantable medical article having a coating comprising
a first component comprising laminin, an active portion thereof, or a binding member thereof, and
a second component comprising an adhesion factor, an active portion thereof, or a binding member thereof,
wherein the coating further comprises a polymeric component, a first group reacted to form a layer comprising the polymeric component, and second groups reacted to individually bond first and second components to the polymeric component.
7 . The implantable medical article of claim 6 wherein the first component comprises laminin having a molecular weight of less than 500 kDa.
8 . The implantable medical article of claim 6 wherein the first component comprises laminin-5.
9 . The implantable medical article of claim 6 wherein the first component comprises the α3 chain of laminin-5.
10 . The implantable medical article of claim 6 wherein the first component comprises the LG3 sequence of the α3 chain of laminin-5.
11 . The implantable medical article of claim 6 wherein the first component comprises a laminin polypeptide sequence selected from PPFLMLLKGSTR, LAIKNDNLVYVY, DVISLYNFKHIY, TLFLAHGRLVFM, LVFMFNVGHKKL, and NSFMALYLSKGR.
12 . The implantable medical article of claim 6 wherein the first component comprises proteinase-modified laminin-5.
13 . The implantable medical article of claim 12 wherein the first component comprises metalloproteinase-modified laminin-5.
14 . The implantable medical article of claim 6 wherein the first component comprises laminin-1
15 . The implantable medical article of claim 6 wherein the second component comprises a collagen, an active portion thereof, or a binding member thereof.
16 . The implantable medical article of claim 15 wherein the second component comprises a collagen I, an active portion thereof, or a binding member thereof.
17 . The implantable medical article of claim 6 comprising a porous portion.
18 . The implantable medical article of claim 17 wherein the porous portion is associated with a graft, sheath, or jacket.
19 . The implantable medical article of claim 17 wherein the porous portion comprises a synthetic hydrophobic polymeric material.
20 . The implantable medical article of claim 19 wherein the porous portion comprises ePTFE.
21 . The implantable medical article of claim 6 wherein the polymeric component comprises a synthetic polymer.
22 . The implantable medical article of claim 21 wherein the synthetic polymer is an acrylamide polymer.
23 . The implantable medical article of claim 6 wherein the first group comprises a photoreactive group.
24 . The implantable medical article of claim 6 wherein the second group comprises an amine-reactive group.
25 . An implantable medical article comprising a stably denucleated porous portion.
26 . The implantable medical article of claim 25 wherein the porous portion comprises a coated layer comprising a synthetic polymer.
27 . The implantable medical article of claim 26 wherein the synthetic polymer comprises pendent photoreactive groups.
28 . A method for preparing an implantable medical article comprising a stably denucleated porous portion comprising the steps of:
denucleating the porous portion; and
forming a coated layer on a surface of the porous portion, the coated layer comprising a synthetic polymer.