Method for filtering particles from a fluid
View Patent ↗Methods for filtering particles from a fluid are disclosed, wherein an array of microstructures defining respective microchannels having respective minimum widths are used to separate the fluid from particles to be filtered. The fluid flows through the minimum widths into the microchannels defined between adjacent microstructures. The particles to be filtered are prevented from passing through the respective minimum widths, resulting in filtration of those particles from the fluid. The microchannels can be provided with gradient characteristics to separate particles in the fluid according to size.
1. A method for filtering particles from a fluid comprising the steps of:
providing an analytical platform comprising: a substrate; an array of microstructures on the substrate; an application surface located opposite said substrate relative to said microstructures; and a plurality of microchannels defined respectively between adjacent ones of said microstructures, said plurality of microchannels each having a first width at a first distance from said substrate and a second width at a second distance from said substrate, said first distance being shorter than said second distance, said second width being a minimum width that is smaller than said first width in each microchannel, said minimum width being equal to or less than a minimum dimension of particles to be filtered out of a fluid;
applying the fluid to said application surface;
using capillary forces to make the fluid enter said plurality of microchannels through the respective minimum widths thereof, wherein the particles to be filtered out of said fluid are prevented from passing through the minimum widths of said plurality of microchannels; and
using at least one of capillary forces, gravity forces, and centrifugal forces to spread the fluid that has passed through the minimum widths of said plurality of microchannels along said microchannels into areas of the analytical platform where no fluid to be filtered was applied to the application surface.
2. A method for filtering particles from a fluid comprising the steps of:
performing the method according to claim 1 at a first area A of the analytical platform; and
transporting in a transport region of the analytical platform the fluid from the first area A to at least a second area B of the analytical platform where a chemical reaction takes place.
3. The method according to claim 2 , further comprising applying a chemical that can react with said fluid to said application surface in area B, and detecting a reaction between said fluid and said chemical.
4. The method according to claim 3 , wherein said reaction is detected by a fluorescence detection scheme.
5. The method according to claim 2 , wherein the analytical platform comprises an array of area A regions, area B regions and transport regions.
6. A method for filtering particles from a fluid comprising the steps of:
providing an analytical platform comprising: a substrate; an array of microstructures on the substrate; an application surface located opposite said substrate relative to said microstructures; and a plurality of microchannels defined respectively between adjacent ones of said microstructures, said plurality of microchannels each having a first width at a first distance from said substrate and a second width at a second distance from said substrate, said first distance being shorter than said second distance, said second width being a minimum width that is smaller than said first width in the respective microchannel, said plurality of microchannels each having gradient characteristics in the minimum width along the channel;
applying the fluid to said application surface;
making the fluid enter said plurality of microchannels through the respective minimum widths thereof, wherein the particles to be filtered out of said fluid are prevented from passing through the minimum widths of said plurality of microchannels and, because of the gradient characteristics, separating the particles according to their size; and
using at least one of capillary forces, gravity forces, and centrifugal forces to spread the fluid that has passed through the minimum widths of said plurality of microchannels along said microchannels into areas of the analytical platform where no fluid to be filtered was applied to the application surface.