Glycosylated interferon alpha obtained from a transgenic chicken
View Patent ↗This invention includes compositions comprising glycosylated human interferon-α derived from a chicken.
1. A composition comprising human interferon-α derived from a chicken wherein the interferon is glycosylated with:
wherein Gal=Galactose,
NAcGal=N-Acetyl-Galactosamine,
NAcGlu=N-Acetyl-Glucosamine, and
SA=Sialic Acid.
2. A pharmaceutical composition comprising the interferon of claim 1 .
3. The pharmaceutical composition of claim 2 wherein the interferon is glycosylated as follows:
4. The interferon of claim 1 wherein the interferon is interferon α2.
5. The interferon of claim 1 wherein the glycosylation is O-linked.
6. The interferon of claim 5 wherein the O-linked glycosylation is at threonine-106.
7. The interferon of claim 1 wherein the interferon comprises SEQ ID NO: 30.
8. A composition comprising human interferon-α derived from a chicken glycosylated with:
wherein Gal=Galactose,
NAcGal=N-Acetyl-Galactosamine,
NAcGlu=N-Acetyl-Glucosamine, and
SA=Sialic Acid.
9. The interferon of claim 8 wherein the interferon is interferon α2.
10. The interferon of claim 8 wherein the glycosylation is O-linked.
11. The interferon of claim 10 wherein the O-linked glycosylation is at threonine-106.
12. The interferon of claim 8 wherein the interferon comprises SEQ ID NO: 30.
13. A human interferon-α comprising a chicken derived glycosylation pattern wherein the glycosylation is not the same as a human interferon-α.
14. The interferon of claim 13 wherein the interferon is interferon α2.
15. The interferon of claim 13 wherein the glycosylation is O-linked.
16. The interferon of claim 15 wherein the O-linked glycosylation is at threonine-106.
17. The interferon of claim 13 wherein the glycosyaltion is produced in a tubular gland cell of the chicken.
18. The interferon of claim 13 wherein the interferon comprises SEQ ID NO: 30.