Operator independent programmable sample preparation and analysis system
View Patent ↗The present invention provides a protocol and apparatus for enriching circulating tumor cells and other rare cells from blood, including debris and other components, from samples with high precision and at high throughput rates. This invention discloses an improved processing system from previously described semi-automated sample processing. The system further reduces operator intervention and hands-on time from prior systems. While this system has general utility in processing diverse materials, the system is configured for sample processing of biological specimens to provide an enriched fraction suitable for detection, enumeration and identification of target cells by appropriate analytical methodologies. The presence and quantities of such target cells in a sample specimen can utilized for screening and detection in disease such as cancer, assessing early stage pre-metastatic cancer, monitoring for disease remission in response to therapy and selection of more effective dose regimens or alternative therapies in case of relapse.
1. A device for processing biological samples suspected to contain rare target bioentities comprising;
a. one or more carriers for receiving and transporting vessels containing said biological samples;
b. one or more carriers for receiving and transporting vessels containing reagents;
c. one or more vessels containing bulk system fluid;
d. one or more fluidic pumping means for manipulating said biological sample and said reagents wherein said pumping means has an aspiration rate between 120 ul/sec to 250 ul/sec to prevent damage to said rare target bioentites linked to paramagnetic particles;
e. one or more magnetic separators incorporating a tube rotation system
f. at least one magnetic incubator having a 70-degree tripole configuration; and
g. one or more programmable controlling means capable of storing and executing commands to control various system components wherein at least one command requires backfluid flushing when aspirating and dispensing said reagents.
2. The device of claim 1 , further comprising one or more electronic means capable of tracking reagent inventory.
3. The device of claim 1 , further comprising one or more electronic means capable of tracking reagent inventory.
4. The device of claim 1 , further comprising one or more electronic means for tracking sample identification.
5. The device of claim 1 , wherein said fluidic pumping means includes one or more non-magnetic alloy probes connected to a pump where said probes are positioned within said vessels using capacitive liquid level sensing and wherein said probes contain small air gaps when used in multiple pick-up methods.
6. The device of claim 5 , further comprising one or more washing means for washing said probes after contacting said biological sample or said reagents.
7. The device of claim 1 , wherein said magnetic separators create a holding force within said vessels containing said biological samples that is greater than shear forces experienced by aspiration of said biological sample from said magnetically collected target bioentity during processing.
8. The device of claim 7 , wherein said magnetic separators create a downward force in addition to said holding force in order to vertically concentrate said magnetically collected target bioentity.
9. The device of claim 8 , further comprising a means for rotating said vessels containing said biological samples while subjected to magnetic forces order to concentrate said magnetically collected target bioentity.
10. The device of claim 1 , wherein said magnetic separators create lateral forces capable of moving magnetically responsive entities through said biological sample.
11. The device of claim 10 , wherein moving said magnetically responsive entities through said biological sample enhances labeling of said entities
12. The device of claim 1 , wherein said target bioentites are selected from a group consisting of epithelial cells, tumor cells, endothelial cells, fungal cells, bacterial cells, cellular debri, cellular components, and combinations thereof.
13. The device of claim 1 , wherein said processing is in preparation of diagnostic image analysis, diagnostic nucleic acid analysis, and combinations thereof.