Cyclosporin analog formulations
View Patent ↗The present invention relates to formulations containing cyclosporin analogs that are structurally similar to cyclosporin A, in particular isomeric mixtures of cyclosporin analogs that are structurally similar to cyclosporin A. The formulations form stable microemulsion preconcentrates and may provide superior drug bioavailability and/or may reduce one or more adverse effects associated with the administration of cyclosporin. Also disclosed are methods for using and preparing the formulations.
1. A pharmaceutical composition comprising:
a) a pharmaceutically effective amount of ISA Tx 247;
b) Vitamin E TPGS;
c) MCT oil;
d) an emulsifier; and
e) ethanol
wherein the pharmaceutical composition forms a clear microemulsion upon contact with an aqueous medium.
2. The pharmaceutical composition of claim 1 wherein the emulsifier is selected from the group consisting of polyoxyethylene(20)sorbitanmonopalmitate and polyoxyethylene(20)sorbitanmonooleate.
3. The pharmaceutical composition of claim 1 , which comprises from about 5% to about 10% by weight of ISA Tx 247, greater than 0% to about 50% by weight of Vitamin E TPGS. greater than 0% to about 50% by weight of MCT oil, greater than 0% to about 50% by weight of emulsifier and about 5% to about 15% by weight of ethanol, wherein the composition is a liquid microemulsion preconcentrate at room temperature.
4. The pharmaceutical composition of claim 3 , wherein the ratio by weight of ISA Tx 247: Vitamin E TPGS: MCT oil: emulsifier: ethanol is about 0.5-1:4:2:2:1.
5. The pharmaceutical composition of claim 3 wherein the emulsifier is polyoxyethylene(20)sorbitanmonopalmitate and further wherein the preconcentrate, when mixed with an aqueous medium in a ratio of about 1 part preconcentrate to about 10 to about 100 parts aqueous medium, forms a clear stable microemulsion solution.
6. The pharmaceutical composition of claim 1 , wherein the bioavailability of ISA Tx 247 is greater than about 30%.
7. The pharmaceutical composition of claim 1 , which is adapted for oral administration.
8. The pharmaceutical composition of claim 1 , which further comprises biocompatible polymers as protective colloids, suspension stabilizers or bulking agents, excipients, binders and carriers.
9. The pharmaceutical composition of claim 8 , wherein the biocompatible polymer is selected from the group consisting of polyoxyethylene glycolated natural or hydrogenated vegetable oils, polyoxyl castor oils, polyoxyethylene-sorbitan-fatty acid esters, polyoxyethylene-polyoxypropylene block co-polymers, lecithins, propylene glycol mono- and di-fatty acid esters, and propylene glycol caprylic-capric acid diester.
10. The pharmaceutical composition of claim 8 , wherein the biocompatible polymer is selected from the group consisting of polyoxyethylene(20)sorbitanmonolaurate, polyoxyethylene(20)sorbitamnonopalmitate, polyoxyethylene(20)sorbitantrioleate, polyoxyethylene(20)sorbitanmonostearate, polyoxyethylene(20)sorbitanmonooleate, polyoxeythylene(20)sorbitantristearate, polyoxyethylene(4)sorbitanmonolaurate, polyoxyethylene(4)sorbitanmonostearate, and polyoxyethylene(5)sorbitanmonooleate.
11. method of producing immunosuppression comprising administering to a subject in need thereof an effective amount of the pharmaceutical composition of claim 1 .
12. The method of claim 11 , wherein the amount of ISA Tx 247 administered is about 0.5 to 3 mg/kg of body weight of said subject per day.
13. The method of claim 11 , wherein the pharmaceutical composition is administered using a schedule selected from the group consisting of once a day and twice a day.
14. The method of claim 11 , wherein the pharmaceutical composition is used to treat an inflammatory or autoinimune disease or condition in said subject.
15. A method of increasing the immunosuppressive effects of ISA Tx 247 by preparing the pharmaceutical composition of claim 1 .
16. The pharmaceutical composition of claim 1 which is a microernulsion preconcentrate.
17. The pharmaceutical composition of claim 16 , which is mixed with an aqueous medium in a ratio of about 1 part preconcentrate to about 10 to about 100 parts aqueous medium.
18. The pharmaceutical composition of claim 17 , wherein the aqueous medium is selected from the group consisting of water, fruit juice, tea, milk and cocoa, wherein the fruit juice is not grapefruit juice.
19. The pharmaceutical composition of claim 18 , wherein the fruit juice is apple juice.
20. The pharmaceutical composition of claim 7 , wherein the oral formulation is encapsulated in a soft gelatin capsule.
21. A method of preparing the pharmaceutical composition of claim 1 comprising mixing the MCT oil, the emulsifier, the ethanol, the Vitamin E TPGS, and the pharmaceutically effective amount of ISA Tx 247 until the ISA Tx 247 is completely dissolved, wherein the composition is a liquid at room temperature.
22. The pharmaceutical composition of claim 1 , wherein the pharmaceutical composition comprises from about 5% to about 10% by weight of ISA Tx 247, from about 20% to about 50% by weight of Vitamin E TPGS, from about 5% to about 20% by weight of MCT oil, from about 5% to about 50% by weight of polyoxyethylene(20) sorbitanmonopalmitate and about 5% to about 15% by weight of ethanol.
23. The pharmaceutical composition of claim 22 , wherein the ratio by weight of ISA Tx 247 Vitamin E TPGS: MCT oil: emulsifier: ethanol is about 0.5-1:4:2:2:1.
24. The pharmaceutical composition of claim 1 , wherein the pharmaceutical composition comprises from about 5% to about 10% by weight of ISA Tx 247, from about 20% to about 50% by weight of Vitamin E TPGS, from about 5% to about 20% by weight of MCT oil, from about 5% to about 50% by weight of polyoxyethylene(20)sorbitamnonooleate and about 5% to about 15% by weight of ethanol.
25. The pharmaceutical composition of claim 24 , wherein the ratio by weight of ISA TX 247: Vitamin E TPGS: MCT oil: emulsifier: ethanol is about 0.5-1:2:4:2:1.
26. The pharmaceutical composition of claim 1 wherein the emulsifier is polyoxyethylene(20)sorbitanmonopalmitate and further wherein the pharmaceutical composition is suitable for subcutaneous or intramuscular administration.
27. A method of preparing the pharmaceutical composition of claim 1 , comprising mixing the MCT oil, the emulsifier, the ethanol, the Vitamin B TPGS, and the pharmaceutically effective amount of ISA Tx 247 until the ISA Tx 247 is completely dissolved.
28. The pharmaceutical composition preconcentrate of claim 1 , wherein the pharmaceutical composition comprises from about 5% to about 10% by weight of ISA Tx 247, from about 20% to about 50% by weight of Vitamin E TPGS from about 5% to about 20% by weight of MCT oil, from about 5% to about 50% by weight of polyoxyethylene(20)sorbitanmonopalmitate and about 5% to about 15% by weight of ethanol.
29. The pharmaceutical composition preconcentrate of claim 28 , wherein the ratio by weight of ISA Tx 247: Vitamin E TPGS: MCT oil: emulsifier: ethanol is about 0.5-1:4:2:2:1.
30. A method of preparing the pharmaceutical composition preconcentrate of claim 1 comprising mixing the MCT oil, polyoxyethylene(20)sorbitanmonopalmitate, the ethanol, the Vitamin B TPGS, and the pharmaceutically effective amount of ISA Tx 247 until the ISA Tx 247 is completely dissolved then mixing the composition into an aqueous medium until a microemulsion preconcentrate solution is formed.
31. A pharmaceutical composition comprising:
a) a pharmaceutically effective amount of ISA Tx 247;
b) Vitamin B TPGS;
c) MCT oil;
d) at least one emulsifier; and
e) ethanol;
wherein the pharmaceutical composition forms a clear microemulsion upon contact with an aqueous medium.