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View Patent ↗The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel promoter nucleotide sequence for the gene encoding ubiquitin in Tripsacum dactyloides , as well as vectors, microorganisms, plants and plant cells having the promoter nucleotide sequence, or variants and fragments thereof. Methods for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein are also provided. The methods include stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
1. An isolated nucleic acid molecule comprising a nucleotide sequence having at least 99% sequence identity to the sequence set forth in SEQ ID NO:2, wherein said nucleotide sequence initiates transcription in a plant cell.
2. An expression cassette comprising the nucleotide sequence of claim 1 operably linked to a heterologous nucleotide sequence of interest.
3. A vector comprising the expression cassette of claim 2 .
4. A plant cell having stably incorporated into its genome the expression cassette of claim 2 .
5. The plant cell of claim 4 , wherein said plant cell is from a monocot.
6. The plant cell of claim 5 , wherein said monocot is maize.
7. The plant cell of claim 4 , wherein said plant cell is from a dicot.
8. A plant having stably incorporated into its genome the expression cassette of claim 2 .
9. The plant of claim 8 , wherein said plant is a monocot.
10. The plant of claim 9 , wherein said monocot is maize.
11. The plant of claim 8 , wherein said plant is a dicot.
12. Transgenic seed of the plant of claim 8 .
13. The plant of claim 8 , wherein the heterologous nucleotide sequence of interest encodes a gene product that confers herbicide, salt, pathogen, or insect resistance.
14. A method for expressing a nucleotide sequence in a plant, said method comprising introducing into a plant cell an expression cassette comprising a promoter operably linked to a heterologous nucleotide sequence of interest, wherein said promoter comprises a nucleotide sequence having at least 99% sequence identity to the sequence set forth in SEQ ID NO:2, wherein said nucleotide sequence initiates transcription in a plant cell, wherein said initiation of transcription results in the expression of the heterologous nucleotide sequence of interest; and,
regenerating a transformed plant from said plant cell, wherein said plant has stably incorporated into its genome said expression cassette.
15. The method of claim 14 , wherein said plant is a dicot.
16. The method of claim 14 , wherein said plant is a monocot.
17. The method of claim 16 , wherein said monocot is maize.
18. The method of claim 14 , wherein said heterologous nucleotide sequence encodes a gene product that confers herbicide or pest resistance.
19. A method for expressing a nucleotide sequence in a plant cell, said method comprising introducing into a plant cell an expression cassette comprising a promoter operably linked to a heterologous nucleotide sequence of interest, wherein said promoter comprises a nucleotide sequence having at least 99% sequence identity to the sequence set forth in SEQ ID NO:2, wherein said nucleotide sequence initiates transcription in a plant cell, wherein said initiation of transcription results in the expression of the heterologous nucleotide sequence of interest.
20. The method of claim 19 , wherein said plant cell is from a monocot.
21. The method of claim 20 , wherein said monocot is maize.
22. The method of claim 19 , wherein said plant cell is from a dicot.
23. The method of claim 19 , wherein the heterologous nucleotide sequence encodes a gene product that confers herbicide or pest resistance.
24. The isolated nucleic acid molecule of claim 1 , wherein said nucleotide sequence is selected from the group consisting of:
(a) the nucleotide sequence set forth in SEQ ID NO: 1, 2, 3, 5 or 6; and,
(b) the nucleotide sequence of the DNA insert of the plasmid deposited as Accession No. NRRL B-30819.
25. The method of claim 14 , wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
(a) the nucleotide sequence set forth in SEQ ID NO:1, 2, 3, 5 or 6; and,
(b) the nucleotide sequence of the DNA insert of the plasmid deposited as Accession No. NRRL B-30819.
26. The method of claim 19 , wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
(a) the nucleotide sequence set forth in SEQ ID NO:1, 2, 3, 5 or 6; and,
(b) the nucleotide sequence of the DNA insert of the plasmid deposited as Accession No. NRRL B-30819.