Process for preparing retrovirus vector for gene therapy
The present invention provides a process for preparing a retrovirus to be expressed at a high titer by specifically transferring a desired foreign gene into target cells. A pseudotyped retrovirus vector having a high titer can be prepared by transferring a DNA construction wherein a promoter, an loxP sequence, a VSV-G gene and a polyA addition signal are arranged in this order is transferred into cells carrying the retrovirus gag and pol gene expression systems, and then transferring a retrovirus vector containing the desired foreign gene thereinto, followed by the treatment with a recombinase.
1 . A DNA construction for regulating the expression of a virus structural protein gene by using a recombinase and its recognition sequence wherein a promoter, the recombinase recognition sequence, a drug resistance gene, a polyA addition signal, the recombinase recognition sequence, the virus structural protein gene and a polyA addition signal are arranged in this order.
2 . A DNA construction for regulating the expression of a foreign gene by using a recombinase and its recognition sequence wherein the LTR of a retrovirus genome and a packaging signal are followed by the recombinase recognition sequence, a drug resistance gene, a polyA addition signal, the recombinase recognition sequence, the foreign gene and LTR arranged in this order.
3 - 33 . (canceled)