Compositions and methods for producing fermentation products and residuals
View Patent ↗The present invention provides compositions and methods designed to increase value output of a fermentation reaction. In particular, the present invention provides a business method of increasing value output of a fermentation plant. The present invention also provides a modified fermentation residual of higher commercial value. Also provided in the present invention are complete animal feeds, nutritional supplements comprising the subject ferment residuals. Further provided by the present invention is a method of performing fermentation, a modified fermentative microorganism and a genetic vehicle for modifying such microoganism.
1. A method of fermentation using carbon-containing material, comprising
(a) mixing a carbon-containing material with a culture comprising genetically modified yeast cells that, in a fermentation reaction, produce an alcohol and a fermentation residual comprising a nutrient selected from the group consisting of an amino acid, a cofactor, a hormone, a protein, a vitamin and a lipid, wherein the yeast cells comprise a recombinant expression vector comprising an exogenous nucleotide sequence encoding a polypeptide and a regulatory sequence that controls the expression of the polypeptide, wherein the expression of said exogenous nucleotide sequence results in increased production of said polypeptide and said nutrient and enhanced concentration of the nutrient _ in the fermentation residual compared with the concentration of the nutrient in a fermentation residual produced by an unmodified corresponding yeast cell when used in the fermentation reaction;
(b) fermenting the culture under conditions suitable for production of the alcohol and under conditions suitable for production the polypeptide and the nutrient;
(c) separating the alcohol from the culture; and
(d) producing the fermentation residual comprising the nutrient.
2. The method of claim 1 , wherein the nutrient is an essential amino acid to at least one domesticated animal and the exogenous polypeptide comprises the essential amino acid.
3. The method of claim 2 , wherein the essential amino acid is selected from the group consisting of lysine, methionine, phenylalanine, threonine, isoleucine, tryptophan, valine, leucine, arginine, taurine and histidine.
4. The method of claim 1 , wherein the expression of the exogenous sequence is under the control of a regulatory sequence selected from the group consisting of a regulatory sequence of a heat shock gene, a regulatory sequence of a toxicity gene and a regulatory sequence of a spore formation gene.
5. The method of claim 1 , wherein the exogenous nucleotide sequence modifies at least one of the structural genes in the nutrient's synthetic pathway.
6. The method of claim 1 , wherein the exogenous nucleotide sequence modifies a regulatory control of the nutrient's synthetic pathway.
7. The method of claim 6 , wherein the synthetic pathway is for an essential amino acid for a domesticated animal.
8. The method of claim 1 , wherein the exogenous nucleotide sequence modifies a structural gene that regulates synthesis of a peptide containing at least one essential amino acid for a domesticated animal.
9. The method of claim 1 , wherein the exogenous nucleotide sequence modifies the nutrient's transport processes out of or into the yeast cell.
10. The method of claim 1 , wherein expression of the exogenous sequence is induced when the fermentation reaction has achieved at least about 50% completion.
11. The method of claim 1 , wherein the nutrient is an essential amino acid to at least one domesticated animal.
12. The method of claim 11 , wherein the essential amino acid is selected from the group consisting of lysine, methionine, phenylalanine, threonine, isoleucine, tryptophan, valine, leucine, arginine, taurine and histidine.
13. The method of claim 1 , wherein the nutrient is a vitamin.
14. The method of claim 13 , wherein the vitamin is selected from the group consisting of vitamin A, vitamin B1, vitamin B2, vitamin B3, vitamin B5, vitamin B6, vitamin B7, vitamin B9, vitamin B12, vitamin C, vitamin D1-D4, a tocopherol, and vitamin K.
15. The method of claim 1 , wherein the nutrient is a lipid.
16. The method of claim 1 , wherein the alcohol is ethanol.
17. The method of claim 1 , wherein the alcohol is selected from the group consisting of methanol, propanol and butanol.
18. The method of claim 1 , wherein the yeast is a Saccharomyces.
19. The method of claim 1 , wherein the carbon-containing material is selected from the group consisting of cellulose, wood chips, vegetables, biomass, excreta, animal wastes, oat, wheat, corn, barley, milo, millet, rice, rye, sorghum, potato, sugar beets, taro, cassaya, fruits, fruit juices, and sugar cane.
20. The method of claim 1 , wherein the fermentation residual comprises distillers dried grains.
21. The method of claim 1 , wherein the fermentation residual comprises distillers dried grains with solubles.
22. The method of claim 1 , further comprising incorporating the fermentation residual into animal feed.
23. The method of claim 16 , further comprising mixing the ethanol with gasoline.
24. The method of claim 1 , wherein the nutrient is produced when fermentation is at least about 95% completed.