Thrombin preparations and process for their production
View Patent ↗The production of a thrombin preparation which is obtained from prothrombin which is, after activation to thrombin without the addition of thromboplastin, purified by a hydrophobic interaction chromatography, it being possible subsequently also to inactivate or remove viruses, is described. Before or after the hydrophobic interaction chromatography it is also possible in addition to carry out a cation exchange chromatography. A thrombin preparation which contains as stabilizer a noncovalently binding inhibitor and to which further stabilizers can be added for stabilization in the liquid state is additionally described.
1. A process for producing a thrombin preparation, which comprises obtaining prothrombin from plasma or a plasma fraction, performing ion exchange chromatography on the prothrombin, activating the prothrombin to thrombin, performing hydrophobic interaction chromatography on the activated thrombin, and performing cation exchange chromatography on the hydrophobic interaction chromatography eluate to yield said thrombin preparation, wherein the hydrophobic interaction chromatography eluate is bound directly to the cation exchange chromatography medium and eluted with a salt gradient, and wherein the yield of thrombin in the thrombin preparation is at least about 70%.
2. The process as claimed in claim 1 , wherein the prothrombin is subjected to virus inactivation or reduction during its production.
3. The process as claimed in claim 1 , wherein the thrombin is subjected to an inactivation or reduction of viruses, before or after the hydrophobic interaction chromatography.
4. The process as claimed in claim 1 , wherein the thrombin preparation is adjusted to a pH of from 5.0 to 8.0.
5. The process as claimed in claim 1 , wherein the thrombin preparation comprises a soluble calcium salt, a sodium chloride as stabilizer, a buffer substance, and at least one of a sugar, a sugar alcohol, an amino acid, a salt of a mono- or polycarboxylic acid, or a salt of a mono- or polyhydroxycarboxylic acid.
6. The process as claimed in claim 1 , wherein a noncovalently binding inhibitor of thrombin activity is added as stabilizer.
7. The process as claimed in claim 6 , wherein the noncovalently binding inhibitor of thrombin activity is benzamidine or p-aminobenzamidine.
8. The process as claimed in claim 1 , wherein a gel with coupled hydrophobic radicals is employed as absorbent for the hydrophobic interaction chromatography.
9. The process as claimed in claim 8 , wherein the hydrophobic radicals are phenyl radicals.
10. The process as claimed in claim 1 , further comprising filtering the thrombin preparation through a membrane with a suitable pore size to remove viruses.