Fluidic medical devices and uses thereof
This invention is in the field of medical devices. Specifically, the present invention provides fluidic systems having a plurality of reaction sites surrounded by optical barriers to reduce the amount of optical cross-talk between signals detected from various reaction sites. The invention also provides a method of manufacturing fluidic systems and methods of using the systems.
1 . An apparatus for detecting an analyte in a biological fluid of a subject, comprising:
a) a sample collection unit for introducing a biological fluid in fluid communication with a plurality of reaction sites;
b) a plurality of reactant chambers carrying a plurality of reactants in fluid communication with said reaction sites,
wherein said plurality of reaction sites comprise a plurality of reactants bound thereto for detecting said analyte; and
c) a system of fluidic channels to allow said biological fluid and said plurality of reactants to flow in said apparatus, wherein at least one channel located between said plurality of reaction sites comprises an optical barrier to reduce the amount of optical cross-talk between said plurality of said reaction sites during detection of said analyte.
2 . The apparatus of claim 1 , further comprising a plurality of waste chambers in fluid communication with at least one of said reaction sites.
3 . The apparatus of claim 1 wherein each channel located between said plurality of reaction sites comprises an optical barrier.
4 . The apparatus of claim 1 wherein the biological fluid is less than about 500 microliters.
5 . The apparatus of claim 1 wherein said optical barrier comprises a nonlinear fluidic channel.
6 . The apparatus of claim 1 wherein the fluid is less than about 50 microliters.
7 . The apparatus of claim 1 , wherein the reactants comprise immunoassay reagents.
8 . The apparatus of claim 7 , wherein said apparatus detects a plurality of analytes, wherein the analytes are identified by distinct signals detectable over a range of 3 orders of magnitude.
9 . The apparatus of claim 7 , wherein the immunoassay reagents detect a polypeptide glycoprotein, polysaccharide, lipid, nucleic acid, and a combination thereof.
10 . The apparatus of claim 7 , wherein the immunoassay reagents detect a member selected from the group consisting of drug, drug metabolite, biomarker indicative of a disease, tissue specific marker, and biomarker specific for a cell or cell type.
11 . The apparatus of claim 1 , wherein the detectable signal is a luminescent signal.
12 . An apparatus for detecting an analyte in a biological fluid of a subject, comprising
a) a sample collection unit for introducing a biological fluid in fluid communication with a plurality of reaction sites, wherein said plurality of reaction sites comprise a plurality of bound reactants for detecting said analyte;
b) a plurality of reactant chambers carrying a plurality of reactants in fluid communication with said reaction sites; and
c) a system of fluidic channels to allow said biological fluid and said plurality of reactants to flow in said apparatus,
wherein said bound reactants in at least one reaction site are unevenly distributed.
13 . The apparatus of claim 12 , wherein said bound reactants in the at least one reaction site are localized around the center of said reaction site.
14 . The apparatus of claim 13 , wherein an outer edge of the at least one reaction site is at a distance sufficiently far from said bound reactants to reduce signals unrelated to the presence of said analyte.
15 . The apparatus of claim 12 , further comprising a waste chamber in fluid communication with said reaction sites.
16 . The apparatus of claim 14 wherein said unbound reactant is coupled to said edge of said reaction site.
17 . A method of manufacturing a fluidic device for detecting an analyte in a biological fluid of a subject, comprising:
a) providing a plurality of layers of a fluidic device;
b) ultrasonically welding said layers together such that a fluidic network exists between a sample collection unit, at least one reactant chamber, at least one reaction site, and at least one waste chamber.
18 . The method of claim 17 wherein said at least one reaction chamber is vacuum sealed.
19 . The method of claim 17 , wherein said fluidic device comprises a system of fluidic channels to allow contact of said biological fluid with reactants in said at least one reaction site.
20 . The method of claim 17 , wherein said reaction site comprises an optical barrier to reduce the amount of optical cross-talk between another reaction site on the fluidic device.