Plants with increased phosphorous uptake
The invention provides plant acid phosphatase coding sequences. Also provided are constructs comprising these sequences, plants transformed therewith and methods of use thereof. In certain aspects of the invention, transgenic plants are provided exhibiting improved phosphorous utilization.
1. An isolated nucleic acid sequence encoding plant acid phosphatase, wherein the nucleic acid sequence is selected from the group consisting of: (a) a nucleic acid sequence encoding the polypeptide of SEQ ID NO:2; (b) a nucleic acid sequence comprising the sequence of SEQ ID NO: 1; (c) a nucleic acid sequence which hybridizes to SEQ ID NO: 1 under wash conditions of 0.15 M NaCl and 70° C. for 10 minutes; (d) a nucleic acid sequence encoding a polypeptide comprising at least 95% sequence identity over the full length of the polypeptide sequence of SEQ ID NO:2; and (e) a nucleic acid sequence fully complementary to the nucleic acid sequence of (a), (b), (c) or (d).
2. The isolated nucleic acid of claim 1 , wherein the nucleic acid is defined as encoding the polypeptide of SEQ ID NO:2.
3. The isolated nucleic acid of claim 1 , wherein the nucleic acid is defined as comprising the nucleic acid sequence of SEQ ID NO:1.
4. A recombinant vector comprising the isolated nucleic acid sequence of claim 1 operably linked to a heterologous promoter.
5. The recombinant vector of claim 4 , further comprising at least one additional sequence chosen from the group consisting of: a regulatory sequence, a selectable marker, a leader sequence and a terminator.
6. The recombinant vector of claim 5 , wherein the additional sequence is a heterologous sequence.
7. The recombinant vector of claim 4 , wherein the promoter is a tissue-specific promoter.
8. The recombinant vector of claim 4 , wherein the promoter is a root-specific promoter.
9. The recombinant vector of claim 4 , defined as an isolated expression cassette.
10. A transgenic plant transformed with the recombinant vector of claim 4 .
11. The transgenic plant of claim 10 , further defined as a monocotyledonous plant.
12. The transgenic plant of claim 10 , further defined as a dicotyledonous plant.
13. The transgenic plant of claim 10 , further defined as a legume.
14. The transgenic plant of claim 10 , further defined as an R 0 transgenic plant.
15. The transgenic plant of claim 10 , further defined as a progeny plant of any generation of an R 0 transgenic plant, wherein the transgenic plant has the nucleic acid sequence from the R 0 transgenic plant.
16. A transgenic seed of the transgenic plant of claim 10 , wherein the seed comprises the nucleic acid sequence.
17. A host cell transformed with the recombinant vector of claim 4 .
18. The host cell of claim 17 , wherein said host cell is a plant cell.