Method to improve plant somatic embryo germination from manufactured seed
The invention provides methods for improving germination of manufactured seeds. In some embodiments, the methods comprise the steps of (a) incubating a plant somatic embryo under suitable conditions for reducing the size of the embryo to produce a compact plant somatic embryo and (b) assembling the compact plant somatic embryo of step (a) into a manufactured seed.
1. A method for improving germination of a manufactured seed, comprising the steps of:
(a) incubating a conifer somatic embryo sufficiently developed to have a shoot end and a root end, at a humidity of between about 90% and about 99.5%, for between about 7 days and 20 days, to reduce the size of the embryo to produce a compact conifer somatic embryo; and
(b) assembling the compact conifer somatic embryo of step (a) into a manufactured seed.
2. The method of claim 1 , wherein the length of the conifer somatic embryo is reduced by about 10% to 50%.
3. The method of claim 1 , wherein the width of the conifer somatic embryo is reduced by about 10% to 50%.
4. The method of claim 1 , wherein the moisture content of the compact conifer somatic embryos is above 60%.
5. The method of claim 1 , wherein the conifer somatic embryo is a loblolly pine somatic embryo.