Instrument for monitoring DNA replication
View Patent ↗An instrument for monitoring replication of DNA is provided.
1. An instrument comprising:
a plurality of spaced-apart reaction regions;
a light source adapted to direct an excitation beam toward the plurality of reaction regions;
a detector disposed to receive emission beams from the reaction regions;
an excitation beam path disposed along an optical path between the light source and one of the reaction regions;
an emission beam path disposed along an optical path between the reaction regions and the detector; and
a lens located in front of the reaction regions, the lens disposed along the excitation beam path and along the emission beam path, the lens configured to direct the excitation beam to more than one of the reaction regions simultaneously.
2. The instrument of claim 1 , further comprising reaction ingredients disposed in each of the plurality of reaction regions, the reaction ingredients comprising components for DNA replication.
3. The instrument of claim 2 , wherein the components for DNA replication comprise DNA primer strands.
4. The instrument of claim 2 , wherein the reaction ingredients further comprise a fluorescent dye.
5. The instrument of claim 2 , wherein the reaction ingredients further comprise fluorescent dye labeled probes.
6. The instrument of claim 1 , wherein the lens is a field lens that provides a telecentric optical system.
7. The instrument of claim 1 , further comprising a plurality of lenses corresponding to respective ones of the plurality of reaction regions, the plurality of lenses being disposed between the lens and the plurality of reaction regions, wherein emission light from at least two of the reaction regions simultaneously passes through the lens and onto the detector.
8. The instrument of claim 1 , further comprising emission beams from at least two of the reaction regions that is produced by the excitation beam, wherein the at least two emission beams simultaneously pass through the lens and onto the detector.
9. An instrument comprising:
a plurality of spaced-apart reaction regions;
a light source adapted to direct an excitation beam toward the plurality of reaction regions;
a focusing optic disposed along an excitation beam path between the light source and the plurality of reaction regions;
a plurality of lenses, each lens being disposed between the focusing optic and a corresponding one of the plurality of reaction regions;
a detector disposed to receive emission beams from the focusing optic; and
an emission beam path disposed from the plurality of reaction regions to the detector;
wherein the excitation beam path and the emission beam path are disposed along a same optical path through the focusing optic;
wherein the light source, the focusing optic, and the lenses together are configured to simultaneously illuminate two or more reaction regions of the plurality of spaced-apart reaction regions.
10. The instrument of claim 9 , further comprising reaction ingredients disposed in each of the plurality of reaction regions, the reaction ingredients comprising components for DNA replication.
11. The instrument of claim 10 , wherein the components for DNA replication comprise DNA primer strands.
12. The instrument of claim 10 , wherein the reaction ingredients further comprise a fluorescent dye.
13. The instrument of claim 10 , wherein the reaction ingredients further comprise fluorescent dye labeled probes.
14. The instrument of claim 9 , wherein each of the plurality of reaction regions comprises a vial including one of the plurality of lenses.
15. The instrument of claim 14 , wherein the focusing optic and the plurality of lenses are disposed to cooperatively effect focusing of the excitation beam into the reaction regions.
16. The instrument of claim 9 , wherein the focusing optic is a field lens that provides a telecentric optical system.
17. An instrument comprising:
an apparatus configured to hold a plurality of spaced-apart reaction regions;
a light source configured to direct an excitation beam toward the plurality of reaction regions;
a detector configured to receive emission beams from the reaction regions;
an excitation beam path disposed from the light source to the plurality of reaction regions;
an emission beam path disposed from the plurality of reaction regions to the detector; and
a lens disposed along the excitation beam path and along the emission beam path, the lens configured to direct the excitation beam to more than one of the reaction regions simultaneously.
18. The instrument of claim 17 , wherein lens is a focusing optic.
19. The instrument of claim 17 , wherein the lens is located in front of, and proximal to, all of the reaction regions.
20. The instrument of claim 17 , further comprising a plurality of lenses corresponding to respective ones of the plurality of reaction regions, the plurality of lenses being disposed between the field lens and the plurality of reaction regions, wherein emission light from at least two of the reaction regions simultaneously passes through the field lens and onto the detector.
21. The instrument of claim 17 , further comprising emission beams from at least two of the reaction regions that is produced by the excitation beam, wherein the at least two emission beams simultaneously pass through the field lens and onto the detector.
22. The instrument of claim 17 , wherein the focusing optic is a field lens that provides a telecentric optical system.
23. The instrument of claim 17 , wherein the components for DNA replication comprise DNA primer strands.
24. The instrument of claim 23 , wherein the reaction ingredients further comprise a fluorescent dye.
25. The instrument of claim 23 , wherein the reaction ingredients further comprise fluorescent dye labeled probes.
26. The instrument of claim 23 , further comprising reaction ingredients disposed in each of the plurality of reaction regions, the reaction ingredients comprising components for DNA replication.