Compositions and methods for human metapneumovirus monoclonal antibodies
View Patent ↗The present invention discloses specific human metapneumovirus monoclonal antibodies. The antibody is at least two-fold less reactive with non-human metapneumoviruses including, but not limited to, respiratory viruses or avian metapneumoviruses. Further, the antibody is at least two-fold more reactive with a human metapneumovirus (i.e., for example, Type A or Type B) than with non-human metapneumoviruses including, but not limited to, respiratory viruses or avian metapneumoviruses. Consequently, these novel antibodies are useful as a clinical diagnostic agent, especially when using fresh nasopharengeal aspirates. The invention also contemplates numerous diagnostic platforms that together with the novel antibodies can support economical, fast, and highly selective detection and identification of clinical inoculum samples.
1. A human metapneumovirus monoclonal antibody panel, comprising a first monoclonal antibody produced by the mouse spleen hybridoma clone C2C 10, a second monoclonal antibody produced by the mouse spleen hybridoma clone F4A1, and a third monoclonal antibody produced by the mouse spleen hybridoma clone CB7F3.
2. The panel of claim 1 , wherein said panel further comprises a respiratory cell sample.
3. The panel of claim 1 , wherein said first, second, and third antibodies are fluorescently labeled.
4. A method of detecting human metapneumovirus, comprising:
a) providing
i) a human metapneumovirus monoclonal antibody panel, comprising a first monoclonal antibody produced by the mouse spleen hybridoma clone C2C10, a second monoclonal antibody produced by the mouse spleen hybridoma clone F4A1, and a third monoclonal antibody produced by the mouse spleen hybridoma clone CB7F3
ii) a biological sample and
iii) a diagnostic platform capable of detecting binding between antibody and sample
b) introducing said biological sample using said diagnostic platform under conditions such that said at least one monoclonal antibody interacts with said sample and
c) detecting binding of at least one monoclonal antibody, wherein binding indicates detection of human metapneumovirus.
5. The method of claim 4 , wherein said monoclonal antibodies are fluorescently labeled.
6. The method of claim 5 , wherein said wherein said diagnostic platform is a direct immunofluorescence assay.
7. The method of claim 4 , wherein said diagnostic platform comprises a cell infection assay.
8. The method of claim 7 , wherein said assay comprises introducing the biological sample into a culture of tMK cells, wherein said tMK cells are contained within a plurality of well plates.
9. The method of claim 8 , further comprising detecting the appearance of cytopathic effects within said tMK cells.
10. The method of claim 4 , wherein said biological sample is concentrated using an elution electrophoresis system.
11. The method of claim 10 , wherein said elution electrophoresis system comprises a porous material under the influence of an electrical field.
12. The method of claim 11 , wherein said porous material is selected from the group consisting of dextran, cellulose, acrylamide, polymeric particles, and silica.
13. The method of claim 4 , wherein said diagnostic platform comprises a solid support.
14. The method of claim 13 , before step (b), binding said antibody to said solid support.
15. The method of claim 13 , wherein said solid support comprises a test strip.
16. The method of claim 15 , wherein said test strip is attached to a dipstick.
17. The method of claim 16 , wherein said dipstick comprises compounds selected from the group consisting of radioactive tracers, enzymes, colloidal metals, and substrates.
18. The method of claim 4 , wherein said diagnostic platform comprises a micro fluidic device.
19. The method of claim 18 , wherein said microfluidic device comprises a ferrofluid, wherein said antibodies are attached to said ferrofluid.
20. The method of claim 18 , wherein said microfluidic device comprises at least one microchannel in fluidic communication with at least one microchamber.