Polypeptides having endoglucanase activity and polynucleotides encoding same
View Patent ↗The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
1. An isolate polypeptide having endoglucanase activity, selected from the group consisting of:
(a) a polypeptide comprising an amino acid sequence having at least 95% sequence identity with the mature polypeptide of SEQ ID NO:2;
(b) a polypeptide encoded by a polynucleotide which hybridizes under high stringency conditions with the full length complementary strand of (i) the mature polypeptide coding sequence of SEQ ID NO:1, or (ii) the genomic DNA sequence comprising the mature polypeptide coding sequence of SEQ ID NO:1; and
(c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity with the mature polypeptide coding sequence of SEQ ID NO:1.
2. The polypeptide of claim 1 , which is encoded by the polynucleotide contained in plasmid pTter7F which is contained in E. coli NRRL B-30837.
3. The polypeptide of claim 1 , wherein the mature polypeptide is amino acids 18 to 336 of SEQ ID NO: 2.
4. The polypeptide of claim 1 , wherein the mature polypeptide coding sequence is nucleotides 52 to 1008 of SEQ ID NO: 1.
5. The polypeptide of claim 1 , further having enzyme activity toward one or more substrates selected from the group consisting of xylan, xyloglucan, arabinoxylan, 1,4-galactan, galactomannan, dextran, and chitin.
6. A method for producing the polypeptide of claim 1 comprising: (a) cultivating a cell, which in its wild-type form is capable of producing the polypeptide, under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide.
7. A method for producing a protein comprising: (a) cultivating a recombinant host cell under conditions conducive for production of the protein, wherein the recombinant host cell comprises a nucleic acid construct comprising a gene encoding a protein operably linked to a nucleotide sequence encoding a signal peptide comprising or consisting of amino acids 1 to 17 of SEQ ID NO: 2, wherein the gene is foreign to the nucleotide sequence; and (b) recovering the protein.
8. A detergent composition comprising the polypeptide of claim 1 and a surfactant.
9. The polypeptide of claim 1 , which comprises an amino acid sequence having at least 95% sequence identity with the mature polypeptide of SEQ ID NO: 2.
10. The polypeptide of claim 9 , which comprises an amino acid sequence having at least 97% sequence identity with the mature polypeptide of SEQ ID NO: 2.
11. The polypeptide of claim 1 , which is encoded by a polynucleotide which hybridizes under high stringency conditions with the full length complementary strand of (i) the mature polypeptide coding sequence of SEQ ID NO:1, or (ii) the genomic DNA sequence comprising the mature polypeptide coding sequence of SEQ ID NO:1.
12. The polypeptide of claim 1 , which is encoded by a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity with the mature polypeptide coding sequence of SEQ ID NO: 1.
13. The polypeptide of claim 12 , which is encoded by a polynucleotide comprising a nucleotide sequence having at least 97% sequence identity with the mature polypeptide coding sequence of SEQ ID NO: 1.