Method of making a biosensor
A method of making a biosensor is provided. The biosensor includes an electrically conductive material on a base and electrode patterns formed on the base, the patterns having different feature sizes. The conductive material is partially removed from the base using broad field laser ablation so that less than 90% of the conductive material remains on the base and that the electrode pattern has an edge extending between two points. A standard deviation of the edge from a line extending between two points is less than about 6 μm
1 . A method of making a biosensor electrode set, comprising:
providing a laser system having a lens and a mask, and
ablating through a portion of a metallic layer with a laser, to form an electrode pattern, the pattern of ablation being controlled by the lens and the mask;
wherein said first metallic layer is on an insulating substrate.
2 . The method of claim 1 wherein said metallic layer comprises copper.
3 . The method of claim 1 wherein said metallic layer comprises at least one member selected from the group consisting of gold, platinum, palladium and iridium.
4 . The method of claim 1 wherein said insulating substrate is a polymer.
5 . The method of claim 1 wherein said pattern has a feature size of less than 100 μm.
6 . The method of claim 1 wherein said pattern has a feature size of less than 75 μm.
7 . The method of claim 1 wherein said pattern has a feature size of 1 μm to 50 μm.
8 . The method of claim 1 wherein the pattern is formed as a microelectrode array.
9 . The method of claim 1 wherein said metallic layer is in contact with said insulating substrate.
10 . The method of claim 9 wherein said metallic layer comprises at least one member selected from the group consisting of gold, platinum, palladium and iridium.
11 . The method of claim 1 further comprising the step of applying a second metallic layer on said metallic layer.
12 . The method of claim 1 wherein said electrode pattern is formed in less than about 0.25 seconds.
13 . The method of claim 1 wherein said electrode pattern is formed in less than about 50 nanoseconds.
14 . The method of claim 1 wherein said electrode pattern is formed in about 25 nanoseconds.
15 . A method of making an electrode set ribbon, comprising:
providing a laser system having a lens and a mask, and
ablating through a portion of a metallic layer with a laser, to form a plurality of electrode patterns, the pattern of ablation being controlled by the lens and the mask,
wherein the metallic layer is on an insulating substrate, and
said electrode set ribbon comprises a plurality of electrode sets.
16 . The method of claim 15 wherein said metallic layer comprises at least one member selected from the group consisting of gold, platinum, palladium and iridium and said metallic layer is in contact with said insulating substrate.
17 . The method of claim 15 wherein said electrode pattern is formed in less than about 50 nanoseconds.
18 . The method of claim 15 wherein said electrode pattern is formed in about 25 nanoseconds.
19 . The method of claim 15 wherein at least one pattern has a feature size of less than 100 μm.
20 . The method of claim 15 wherein at least one pattern has a feature size of 1 μm to 50 μm.
21 . A method of making a sensor strip, comprising:
providing a laser system having a lens and a mask,
forming an electrode set by ablating through a portion of a first metallic layer with a laser, a pattern of ablation being controlled by the lens and the mask;
wherein said first metallic layer is on an insulating substrate, and
cutting said substrate, to form a strip.
22 . The method of claim 21 further comprising the step of applying a dielectric on a portion of said metallic layer.
23 . The method of claim 21 further comprising the step of applying a reagent on a portion of said electrode set.
24 . The method of claim 21 wherein said metallic layer comprises at least one member selected from the group consisting of gold, platinum, palladium and iridium.
25 . The method of claim 21 wherein the electrode set is formed in less than about 50 nanoseconds.
26 . The method of claim 21 wherein the electrode set is formed in about 25 nanoseconds.
27 . The method of claim 21 wherein at least one pattern has a feature size of less than 100 μm.
28 . The method of claim 21 wherein at least one pattern has a feature size of 1 μm to 50 μm.
29 . A method of making a biosensor electrode set, comprising:
providing a laser system having a lens and a mask, and
ablating through a portion of a first metallic layer with a laser, to form an electrode pattern, the pattern of ablation being controlled by the lens and the mask,
wherein said metallic layer is on an insulating base.
30 . The method of claim 29 wherein the electrode pattern is formed in less than about 50 nanoseconds.
31 . The method of claim 29 wherein said electrode pattern is formed in about 25 nanoseconds.
32 . The method of claim 31 wherein the metallic layer is gold.
33 . The method of claim 31 wherein said insulating base is a polymer.
34 . The method of claim 31 wherein said pattern has a feature size of less than 100 μm.
35 . The method of claim 31 wherein said pattern has a feature size of less than 75 μm.
36 . The method of claim 31 wherein said pattern has a feature size of less than 20 μm.
37 . A method of making a biosensor strip, comprising:
providing a laser system having at least a laser source and a mask, and
forming an electrode set by ablating through a portion of a metallic layer with a laser, a pattern of ablation being controlled by the mask,
wherein said metallic layer is on an insulating base.
38 . The method of claim 37 further comprising the step of applying a reagent on a portion of said electrode set.
39 . The method of claim 37 wherein the electrode set is formed in less than about 200 nanoseconds.
40 . The method of claim 37 wherein the electrode set is formed in less than about 50 nanoseconds.
41 . The method of claim 37 wherein the electrode set is formed in about 25 nanoseconds.
42 . The method of claim 37 wherein the electrode set is formed by a single pulse of laser light from the laser.
43 . The method of claim 37 wherein the electrode set is formed by pulses of laser light from the laser.
44 . A method of making a biosensor, the method comprising the steps of:
providing an electrically conductive material on a base, and
forming a pre-determined electrode pattern on the base using laser ablation through a mask, the mask having a mask field with at least one opaque region and at least one window formed to allow a laser beam to pass through the mask and to impact predetermined areas of the electrically conductive material.
45 . The method of claim 44 wherein the windows are configured in a window pattern identical in geometry to the predetermined electrode pattern.
46 . The method of claim 44 wherein the electrode set is formed in less than about 200 nanoseconds.
47 . The method of claim 44 wherein the electrode set is formed in less than about 50 nanoseconds.
48 . The method of claim 44 wherein the electrode set is formed in about 25 nanoseconds.
49 . The method of claim 44 wherein the electrode pattern is formed by a single pulse of laser light from the laser.
50 . The method of claim 44 wherein the electrode pattern is formed by pulses of laser light from the laser.
51 . The method of claim 44 wherein the forming step includes removing at least 2% of the conductive material from the base.
52 . The method of claim 44 wherein at least 5% of the conductive material is removed from the base.
53 . The method of claim 44 wherein at least 90% of the conductive material is removed from the base.