Spectrograph calibration using known light source and Raman scattering
View Patent ↗A method is provided for calibrating a spectrometer device used for Raman scattering analysis. A predetermined dispersion curve for a diffraction grating or spectrograph of the spectrometer device is modified based on spectrum data associated with detected dispersed light from a calibration light source to produce a modified dispersion curve. The wavelength of a Raman light source on a light detection device is determined. Calibration data for the spectrometer device is computed from the Raman line peak positions for the first chemical, the wavelength on the detection device of the Raman light source and the modified dispersion curve.
1. A method for calibrating a spectrometer device, comprising:
a. activating a calibration light source to emit light that is coupled to a light dispersing element;
b. detecting dispersed light from the light dispersing element to produce first spectrum data;
c. modifying a dispersion curve for the light dispersing element based on said first spectrum data to produce a modified dispersion curve;
d. detecting dispersed light produced as a result of activation of a light source that causes Raman scattering to produce second spectrum data;
e. determining spectral line peak positions for a first chemical and for a second chemical in said second spectrum data; and
f. computing calibration data for the spectrometer device from the spectral line peak positions for said first chemical, the spectral line peak positions for said second chemical, the known Raman wavenumber shifts for said first chemical and for said second chemical and said modified dispersion curve.
2. The method of claim 1 , wherein (c) modifying comprises producing the modified dispersion curve in wavelength space.
3. The method of claim 1 , wherein (c) modifying comprises shifting and expanding or contracting the dispersion curve for the light dispersing element to produce said modified dispersion curve based on peak pixel positions for the calibration light source.
4. The method of claim 3 , wherein (f) computing comprises computing said calibration data in wavenumber space based further on a relationship between wavelengths and wavenumber, thereby fitting the modified dispersion curve to an operating wavelength of the light source that causes Raman scattering.
5. The method of claim 1 , wherein (e) determining comprises determining spectral line peak positions for nitrogen as said first chemical and spectral line positions for oxygen as said second chemical.
6. The method of claim 1 , wherein the first chemical is nitrogen and the second chemical is oxygen, and further comprising monitoring spectrum data when the spectrometer device is in normal use; and determining whether the line peak positions for nitrogen has drifted more than a predetermined number of pixels from calibrated peak positions for nitrogen determined from said monitoring performed over a period of time, and further comprising repeating (a) through (f) when the spectral line peak positions for nitrogen are more than said predetermined number of pixels from said calibrated peak positions for nitrogen.
7. The method of claim 6 , and further comprising adjusting said calibration data so as to keep the spectral line peak positions of nitrogen in the second spectrum data within a predetermined number of pixels of the calibrated line peak positions for nitrogen.
8. The method of claim 1 , wherein (a) activating comprises activating a calibration light source that is part of said spectrometer device.
9. The method of claim 1 , wherein (e) comprising determining spectral line peak positions of nitrogen as said first chemical.
10. The method of claim 1 , wherein (d) detecting comprises detecting Raman scattered light associated with a reference sample, and wherein said reference sample is a solid, liquid or gas.
11. The method of claim 10 , wherein (d) detecting comprises detecting Raman scattered light associated with air.
12. A method for calibrating a spectrometer device used for Raman scattering analysis, comprising:
a. modifying a dispersion curve for a light dispersing element of the spectrometer device based on first spectrum data associated with dispersed light detected by a detection device to produce a modified dispersion curve;
b. determining line peak positions for a first chemical and a second chemical in second spectrum data associated with Raman scattered light detected by said detection device; and
c. computing calibration data for the spectrometer device from the line peak positions for said first chemical and for said second chemical, known Raman wavenumber shifts for said first chemical and for second chemical and said modified dispersion curve.
13. The method of claim 12 , wherein (a) modifying comprises shifting, expanding or contracting the dispersion curve for the light dispersing element to produce said modified dispersion curve based on peak pixel positions for a calibration light source that is used to create the first spectrum data from which the modified dispersion curve is produced.
14. The method of claim 12 , wherein (c) computing comprises computing said calibration data in wavenumber space based further on a relationship between wavelengths and wavenumber, thereby fitting the modified dispersion curve to a wavelength of a light source that causes the Raman scattering light.
15. The method of claim 12 , wherein (b) determining comprises determining spectral line peak positions for nitrogen as said first chemical and oxygen as said second chemical.
16. The method of claim 12 , wherein the first chemical is nitrogen and the second chemical is oxygen, and further comprising monitoring spectrum data when the spectrometer device is in normal use; and determining whether the line peak positions for nitrogen has drifted more than a predetermined number of pixels from calibrated peak positions for nitrogen determined from monitoring over a period of time, and further comprising repeating (a) through (c) when the spectral line peak positions for nitrogen are more than said predetermined number of pixels from said calibrated peak positions for nitrogen.
17. The method of claim 16 , and further comprising adjusting said calibration data so as to keep the spectral line peak positions of nitrogen in the second spectrum data within a predetermined number of pixels from said calibrated peak positions for nitrogen.
18. The method of claim 12 , wherein (b) determining comprises detecting Raman scattered light associated with a reference sample, and wherein said reference sample is a solid, liquid or gas.
19. The method of claim 18 , wherein detecting comprises detecting Raman scattered light associated with air.
20. A spectrometer device, comprising:
a. a light dispersing element;
b. a detector that detects light dispersed by said light dispersing element;
c. a calibration light source;
d. a light source suitable for causing Raman scattering; and
e. a control unit coupled to said detector, wherein said control unit calibrates the spectrometer device by:
i. modifying a dispersion curve for the light dispersing element based on first spectrum data associated with detected dispersed light from said calibration light source, thereby producing a modified dispersion curve;
ii. determining spectral line peak positions for a first chemical and for a second chemical in second spectrum data produced by the detector as result of activation of said light source suitable for causing Raman scattering; and
iii. computing calibration data for the spectrometer device from the line peak positions for said first chemical and for said second chemical, known Raman wavenumber shifts for said first chemical and said second chemical and said modified dispersion curve.
21. The spectrometer device of claim 20 , wherein said control unit modifies said dispersion curve of the light dispersing element by shifting, expanding or contracting data representing the dispersion curve for the light dispersing element to produce said modified dispersion curve based on peak pixel positions for a calibration light source that is used to create the first spectrum data.
22. The spectrometer device of claim 21 , wherein said control unit computes said calibration data in wavenumber space based further on a relationship between wavelengths and wavenumber thereby fitting the modified dispersion curve to a wavelength of the light source suitable for causing Raman scattering.
23. The spectrometer device of claim 20 , wherein said control unit determines spectral line peak positions for nitrogen as said first chemical and spectral line peak positions for oxygen as said second chemical.
24. The spectrometer device of claim 20 , wherein the first chemical is nitrogen and the second chemical is oxygen, and wherein the control unit monitors spectrum data when the spectrometer device is in use and determines whether the line peak positions for nitrogen has drifted more than a predetermined number of pixels from calibrated peak positions for nitrogen determined from monitoring spectrum data over a period of time, and further comprising repeating said (i) modifying, (ii) determining and (iii) computing when the spectral line peak positions for nitrogen are more than said predetermined number of pixels from said calibrated peak positions for nitrogen.