Tumor necrosis factor related ligand
View Patent ↗Nucleic acids that encode Tumor Necrosis Factor Related Ligand (TRELL), a novel member of the tumor necrosis factor family (TNF), modified TRELL, are described.
1. A substantially pure nucleic acid comprising a sequence encoding a polypeptide comprising an amino acid sequence that is at least 90% identical to amino acids 139 to 284 of SEQ ID NO:4, wherein the polypeptide is capable of binding to an HT-29 colon carcinoma cell and is cytotoxic to said carcinoma cell.
2. The nucleic acid of claim 1 , wherein the amino acid sequence is at least 95% identical to amino acids 139 to 284 of SEQ ID NO:4.
3. The nucleic acid of claim 1 , wherein the amino acid sequence is at least 90% identical to amino acids 81 to 284 of SEQ ID NO:4.
4. The nucleic acid of claim 3 , wherein the amino acid sequence is at least 95% identical to amino acids 81 to 284 of SEQ ID NO:4.
5. The nucleic acid of claim 3 , wherein the amino acid sequence is amino acids 81 to 284 of SEQ ID NO:4 with one amino acid substitution, deletion or insertion.
6. The nucleic acid of claim 3 , wherein the amino acid sequence is amino acids 81 to 284 of SEQ ID NO:4 with one conservative amino acid substitution.
7. The nucleic acid of claim 1 , wherein the amino acid sequence is at least 90% identical to amino acids 36 to 284 of SEQ ID NO:4.
8. The nucleic acid of claim 7 , wherein the amino acid sequence is at least 95% identical to amino acids 36 to 284 of SEQ ID NO:4.
9. The nucleic acid according to claim 1 , wherein said polypeptide comprises an amino acid tag sequence.
10. The nucleic acid according to claim 1 , wherein the encoded polypeptide comprises a type I or type II leader sequence.
11. The nucleic acid according to claim 1 , operably linked to an expression control sequence.
12. A nucleic acid vector that comprises the nucleic acid of claim 1 .
13. An isolated host cell transformed with the nucleic acid according to claim 1 .
14. The host cell according to claim 13 , wherein said host cell is a mammalian cell.
15. The host cell according to claim 14 , wherein said mammalian cell is a human cell.
16. A method of producing a polypeptide in an isolated host cell, the method comprising:
(a) providing an isolated host cell that contains a vector comprising the nucleic acid of claim 1 , and;
(b) maintaining the isolated host cell under conditions wherein the nucleic acid is expressed, to thereby produce the polypeptide in the isolated host cell.
17. The method of claim 16 , wherein the host cell is prokaryotic.
18. The method of claim 17 further comprising isolating said polypeptide produced by said host cell to obtain a substantially pure polypeptide.
19. The method of claim 16 , wherein the host cell is eukaryotic.
20. The method of claim 19 further comprising isolating said polypeptide produced by said host cell to obtain a substantially pure polypeptide.
21. A substantially pure nucleic acid comprising a sequence encoding a polypeptide comprising an amino acid sequence that is at least 90% identical to amino acids 139 to 284 of SEQ ID NO:4.
22. The nucleic acid of claim 21 , wherein the amino acid sequence is at least 90% identical to amino acids 81 to 284 of SEQ ID NO:4.
23. The nucleic acid of claim 21 , wherein the amino acid sequence is at least 90% identical to amino acids 36 to 284 of SEQ ID NO:4.